CYP3A4 Polyclonal Antibody

货号：PA1-343

规格：100 µL

价格：5468

产品类型：抗体和染料

品牌：Thermo Fisher

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	单抗	同型对照：
浓度：		用法：	1:1,000-1:5,000(WB)

产品详细信息PA1-343 detects cytochrome P450 (P450) 3A4 from human and rat tissues and cells. This antibody detects, to a lesser extent, other P450 isoforms.PA1-343 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~46 kDa protein representing P450 3A4 from rat liver extracts.The PA1-343 immunogen is purified, His-tagged, full length human P450 3A4 fusion protein.靶标信息This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.

数据

CYP3A4 Antibody (PA1-343) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of Mouse Liver (Lane 1) and Rat Liver (Lane 2). The blots were probed with Anti-Cytochrome P450 3A4 Rabbit Polyclonal Antibody (Product # PA1-343, 1:1000-1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 57 kDa band corresponding to Cytochrome P450 3A4 was observed across tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1.Acta pharmaceutica Sinica. BThe 3'-untranslated region contributes to the pregnane X receptor (PXR) expression down-regulation by PXR ligands and up-regulation by glucocorticoids."Published figure using CYP3A4 polyclonal antibody (Product # PA1-343) in Western Blot"2.Toxicology in vitro : an international journal published in association with BIBRAIn vitro toxicological evaluation of NCS-382, a high-affinity antagonist of γ-hydroxybutyrate (GHB) binding."PA1343 was used in western blot to test the toxicity of The gamma-hydroxybutyric acid receptor antagonist NCS-382 in HepG2 and primary hepatocyte cells"3.PloS oneLocalization and functional characterization of the rat Oatp4c1 transporter in an in vitro cell system and rat tissues."Published figure using CYP3A4 polyclonal antibody (Product # PA1-343) in Western Blot"4.Toxicologic pathologyEffects of lersivirine on canine and rodent thyroid function."PA1-343 was used in immunohistochemistry to study the effects of the non-nucleotide reverse transcriptase inhibitor lersivirine on thyroid function in animals"

技术参数

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