Chromogranin A Monoclonal Antibody (SP12)

货号：MA5-14536

规格：500 µL

价格：4880

产品类型：免疫组化一抗

品牌：Thermo Fisher

物种：人/大鼠

宿主：兔

抗体亚型：IgG

克隆号： SP12

荧光染料：其它

类型:	单抗	同型对照：
浓度：		用法：	1:500-1:5,000 (WB)；1:100(IHC (P)

产品详细信息MA5-14536 targets Chromogranin A in WB, and IHC (P) applications and shows reactivity with Human and Rat samples. This antibody does not cross-react with mouse tissues in IHC applications.The MA5-14536 immunogen is recombinant protein encoding human chromogranin A.靶标信息Chromogranin A (CHGA) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocine cells. CHGA is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides act as autocine or paracrine negative modulators of the neuroendocrine system. CHGA is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

数据

Chromogranin A Antibody (MA5-14536) in IHC (P)Immunohistochemistry analysis of Chromogranin A showing staining in the cytoplasm of paraffin-treated human small intestine tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Chromogranin A monoclonal antibody (Product # MA5-14536) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

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参考文献：

1.Nature communicationsIrp2 regulates insulin production through iron-mediated Cdkal1-catalyzed tRNA modification."Published figure using Chromogranin A monoclonal antibody (Product # MA5-14536) in Western Blot"2.Balkan medical journalThe Ewing's Sarcoma Family of Tumors of Urinary Bladder: A Case Report and Review of the Literature."MA514536 was used in immunohistochemistry to discuss the characteristics of Ewing's Sarcoma family of tumors of urinary bladder"3.Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologieInsulinoma diagnosed as drug-refractory epilepsy in an adolescent boy: a case report."MA5-14536 was used in immunohistochemistry to report on a case of pediatric insulinoma"AuthorsHorváth E,Gozar H,Chira L,Dunca I,Kiss E,Pávai Z4.EndoscopyEvaluation of Ki-67 index in EUS-FNA specimens for the assessment of malignancy risk in pancreatic neuroendocrine tumors."MA5-14536 was used in immunohistochemistry to study the malignancy risk of pancreatic neuroendocrine tumors based on the Ki67 index of endoscopic ultrasound-guided fine-needle aspiration samples"AuthorsHasegawa T,Yamao K,Hijioka S,Bhatia V,Mizuno N,Hara K,Imaoka H,Niwa Y,Tajika M,Kondo S,Tanaka T,Shimizu Y,Kinoshita T,Kohsaki T,Nishimori I,Iwasaki S,Saibara T,Hosoda W,Yatabe Y5.Contemporary oncology (Poznan, Poland)Histological characterisation and prognostic evaluation of 62 gastric neuroendocrine carcinomas."MA514536 was used in immunohistochemistry - paraffin section to find prognostic factors for gastric neuroendocrine carcinoma"AuthorsDeng Y,Chen X,Ye Y,Shi X,Zhu K,Huang L,Zhang S,Ying M,Lin X

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