CD36 Monoclonal Antibody (185-1G2)

货号：MA5-14112

规格：500 µL

价格：4880

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号： 185-1G2

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.2 mg/mL	用法：	1:10-1:100 (ICC/IF)；0.5-1 µg/test(Flow)

产品详细信息MA5-14112 targets CD36 in FACS, immunofluorescence, and immunohistochemistry (frozen) applications and shows reactivity with human and mouse samples.The MA5-14112 immunogen is stimulated human leukocytes.靶标信息CD36 (fatty acid translocase, FAT) is an 88 kDa, ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similar to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids. CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signaling and adapter molecules. CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. Further, CD36 may function as a cell adhesion molecule and directly mediates cyto-adherence of Plasmodium falciparum parasitized erythrocytes. Mutations in the CD36 gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the CD36 protein have been found.

数据

CD36 Antibody (MA5-14112) in ICC/IFImmunofluorescent analysis of CD36 (green) showing staining in the membrane and cytoplasm of THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD36 monoclonal antibody (Product # MA5-14112) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x

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参考文献：

1.NatureSR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis."Published figure using CD36 monoclonal antibody (Product # MA5-14112) in Western Blot"2.Biology of reproductionHistopathologies, immunolocalization, and a glycan binding screen provide insights into Plasmodium falciparum interactions with the human placenta."MA5-14112 was used in immunohistochemistry to study the mechanisms underlying the interaction of Plasmodium falciparum with the human placenta"3.The Journal of pathologyDifferential expression of a gene signature for scavenger/lectin receptors by endothelial cells and macrophages in human lymph node sinuses, the primary sites of regional metastasis."MA5-14112 was used in immunohistochemistry To study the molecular mechanism for the arrest of metastatic tumour cells in human lymph node sinuses"4.PloS oneEphrinA1-Fc attenuates myocardial ischemia/reperfusion injury in mice."MA5-14112 was used in Western Blotting to suggest that ephrinA1-Fc enhances the capacity of the heart to withstand an ischemic insult."5.The Journal of biological chemistryCD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1."MA5-14112 was used in Immunohistochemistry to show ligation of either CD36 or CD47 is sufficient to inhibit NO-stimulated vascular cell responses and cGMP signaling, but only CD47 is necessary for this activity of thrombospondin-1 at physiological concentrations."

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