CD44 Monoclonal Antibody (MEM-85), FITC

货号：MHCD4401

规格：0.5 mL

价格：3402

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG2b

克隆号： MEM-85

荧光染料：FITC

类型:	单抗	同型对照：
浓度：		用法：	Assay-Dependent(Flow)

产品详细信息Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.靶标信息CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

数据

CD44 Antibody (MHCD4401) in FlowCell death & disease 2017 -Figure 3 Induction of tumorigenic stem cell-like properties in infected host cell. ( a ) Immunofluorescent staining of stem cell (SC) marker CD44. Scale bar=2 mu m. ( b ) FACS analysis of CD44 expression. =iMFI. ( c ) Protein levels of SC markers CD44 and CD133. ( d ) Sphere forming ability, showing (i) morphology, (ii) quantitative number and (iii) arbitrary size of spheroids. Scale bar=100 mu m. ( e ) mRNA levels of multipotency transcription factors and SC markers in adherent cells. ( f ) Self-renewing ability of microbe-induced SCs as measured by primary, secondary and tertiary spheroid formation. ( g ) Protein levels of pNF- kappa B and beta -catenin in spheroids. ( h ) mRNA levels of multipotency transcription factors and SC markers in spheroids. ( i ) Protein levels of SC markers in tumorspheres. ( j ) Tumorigenic ability of CD44 high and CD44 low cells of infected culture, showing (i) morphology and (ii) quantitative size. Scale bar=500 mu m. Data=Mean+-S.D. ( d , e , h ) and Mean+-S.E.M. ( j ) for three experiments; * P <0.05, ** P <0.01 and *** P <0.001 versus control. US, Unstained; iMFI ()=integrated Mean Fluorescence Intensity

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参考文献：

1.Experimental hematology & oncologyLoss of quiescence and self-renewal capacity of hematopoietic stem cell in an in vitro leukemic niche."MHCD4401 was used in flow cytometry to assess the human hematopoietic stem cell self-renewal potential and quiescence in an in vitro leukemic niche without leukemic cells"AuthorsVanegas NP,Vernot JP2.Oncology reportsAssessing stemness and proliferation properties of the newly established colon cancer 'stem' cell line, CSC480 and novel approaches to identify dormant cancer cells."MHCD4401 was used in Flow cytometry/Cell sorting to conclude that the CSC480 cell line was an important model to be used in unravelling the underlying mechanisms that control fast-dividing and partially self-renewing stem cells (SCs) that may give rise to cancer."3.Cell death & diseaseInduction of intestinal stemness and tumorigenicity by aberrant internalization of commensal non-pathogenic E. coli."MHCD4401 was used in flow cytometry to observe that chronic presence of internalized Escherichia coli leads to enhanced oncogenicity in colon cancer cells"4.PloS oneSusceptibility of human placenta derived mesenchymal stromal/stem cells to human herpesviruses infection."MHCD4401 was used in flow cytometry to assess the susceptibility of fetal membranes-derived mesenchymal stromal/stem cells to all members of the human Herpesviridae family."

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