Nestin Monoclonal Antibody (10C2)

货号：MA1-110

规格：100 µg

价格：5467

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1

克隆号： 10C2

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	1:500-1:1,500(WB)；1:20-1:100(IHC (P))；Assay-dependent (IHC (F))；1:100(ICC/IF)；1 µg/test (Flow)

产品详细信息MA1-110 has been successfully utilized in Western Blot, immunocytochemistry, flow cytometry, and immunofluorescence applications in human samples. MA1-110 also acts a neuronal stem cell marker and detects a band in Western blot at ~250 kDa.靶标信息Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

数据

Nestin Antibody (MA1-110) in ICC/IFImmunofluorescent analysis of Nestin (green) in human neural stem cells derived from PD-3 iPSCs using Gibco® PSC Neural Induction Medium (Product # A1647801). The cells were fixed and permeabilized using Image-IT® Fixation/Permeabilization kit (Product # R37602), and blocked with the including blocking buffer for one hour at room temperature. Cells were stained with a Nestin monoclonal antibody (Product # MA1-110) at a dilution of 1:100 in blocking buffer for 3 hours at room temperature, and then incubated with a DyLight488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 1 hour at room temperature. Nucleus DNA (blue) was stained with DAPI (Product # D1306). Images were taken on an EVOS® FLoid® Cell Imaging Station at 10X magnification.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Frontiers in cell and developmental biologyCPNE7-Induced Autophagy Restores the Physiological Function of Mature Odontoblasts."Published figure using Nestin monoclonal antibody (Product # MA1-110) in Western Blot"2.Histochemistry and cell biologyAndrogen receptors and gender-specific distribution of alkaline phosphatase in human thyroid cartilage."MA1-110 was used in immunohistochemistry to study gender differences in the localization of android receptors and alkaline phosphatase in human thyroid cartilage"3.Human pathologyComparative immunomorphology of testicular Sertoli and sertoliform tumors."MA1110 was used in immunohistochemistry - paraffin section to develop methods to distinguish Sertoli cell tumors from sertoliform tumors"AuthorsMesa H,Gilles S,Datta MW,Murugan P,Larson W,Dachel S,Manivel JC4.PloS oneEmbryonic stem cells markers SOX2, OCT4 and Nanog expression and their correlations with epithelial-mesenchymal transition in nasopharyngeal carcinoma."MA1-110 was used in immunohistochemistry - paraffin section to assess the expression and prognostic impact of embryonic stem cell markers in nasopharyngeal carcinoma patients"5.Frontiers in aging neuroscienceNovelVariant Linked to Amyotrophic Lateral Sclerosis Risk and Clinical Phenotype."Published figure using Nestin monoclonal antibody (Product # MA1-110) in Immunocytochemistry"

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-44068.htm