Bio-Rad MCA1226SBV710 CD8抗体,Mouse anti Human CD8:StarBright Violet 710-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1226SBV710 CD8抗体,Mouse anti Human CD8:StarBright Violet 710

2024-10-23

Mouse anti Human CD8:StarBright Violet 710

货号：MCA1226SBV710

规格：100 Tests/0.5ml

价格：4106

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD8

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：LT8

荧光染料：StarBright Violet 710

产品介绍
Mouse anti Human CD8 antibody, clone LT8recognizes the human CD8 cell surface glycoprotein expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells.The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells.
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to StarBright Violet 710 - liquid
Species Cross-Reactivity	Target Species	Cross Reactivity
Marmoset	√
Chimpanzee	√
Cynomolgus monkey	√
Red-bellied Tamarin	√
N.B. Antibody reactivity and working conditions may vary between species.
Preparation	Purified IgG prepared by ion exchange chromatography
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 0.1% Pluronic F68 0.1% PEG 3350 0.05% Tween 20
Immunogen	Normal human peripheral blood lymphocytes.
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
StarBright Violet 710	401	713
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Store at +4°C. DO NOT FREEZE.This product should be stored undiluted.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 5ul of the suggested working dilution to label 106cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
相关产品
Description	Product Code	Applications	Pack Size
Human Seroblock	BUF070A	F	50 Test
Human Seroblock	BUF070B	F	200 Test
数据
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 710 conjugated Mouse anti Human CD8 (MCA1226SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
文献
1. Nelson, M.et al.(2010) Characterization of lethal inhalational infection withFrancisella tularensisin the common marmoset (Callithrix jacchus).J Med Microbiol. 59: 1107-13.2. Gibbings, D.J.et al.(2007) CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses.BMC Immunol. 8: 12.3. Junker, A.et al.(2007) Multiple sclerosis: T-cell receptor expression in distinct brain regions.Brain. 130: 2789-99.4. Held, K.et al.(2011) Expression of herpes simplex virus 1-encoded microRNAs in human trigeminal ganglia and their relation to local T-cell infiltrates.J Virol. 85 (19): 9680-5.5. Hood SPet al.(2014) Changes in immune cell populations in the periphery and liver of GBV-B-infected and convalescent tamarins (Saguinus labiatus).Virus Res. 179: 93-101.6. Nelson, M. & Loveday, M. (2014) Exploring the innate immunological response of an alternative nonhuman primate model of infectious disease; the common marmoset.J Immunol Res. 2014: 913632.7. Manivannan, K.et al.(2016) CADM1/TSLC1 Identifies HTLV-1-Infected Cells and Determines Their Susceptibility to CTL-Mediated Lysis.PLoS Pathog. 12 (4): e1005560.8. Gross, C.C.et al.(2016) Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation.Proc Natl Acad Sci U S A. 113 (21): E2973-82.9. Dunham, J.et al.(2016) Blockade of CD127 Exerts a Dichotomous Clinical Effect in Marmoset Experimental Autoimmune Encephalomyelitis.J Neuroimmune Pharmacol. 11 (1): 73-83.10. Bughani, U.et al.(2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.PLoS One. 12 (7): e0180088.11. Philippens, I.H.et al.(2017) Acceleration of Amyloidosis byInflammation in the Amyloid-Beta Marmoset Monkey Model of Alzheimer's Disease.J Alzheimers Dis. 55 (1): 101-113.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Bio-Rad MCA1226SBV710 CD8抗体,Mouse anti Human CD8:StarBright Violet 710

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