Bio-Rad MCA1267F CD4抗体,Mouse anti Human CD4:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1267F CD4抗体,Mouse anti Human CD4:FITC

2024-10-23

Mouse anti Human CD4:FITC

货号：MCA1267F,MCA1267FT

规格：0.1 mg,25 µg

价格：1080,425

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD4

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：RPA-T4

荧光染料：FITC

Mouse IgG1 Negative Control:FITC

产品介绍
Mouse anti human CD4 antibody, clone RPA-T4 recognizes human CD4, a 55 kDa cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule.Mouse anti human CD4 antibody, clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation (Piatier-Tonneau et al, 1997). Bio-Rad recommend the use of Mouse anti Human CD4:Low Endotoxin (MCA1267EL) for this purpose.
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	MCA1267F: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant MCA1267FT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin
Immunogen	Human PHA blasts
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 10⁶cells or 100ul whole blood
数据
Figure A. RPE conjugated mouse anti human CD3 (MCA463PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti human CD3 (MCA463PE) and FITC conjugated mouse anti human CD4 (MCA1267F). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:FITC	MCA928F	F	100 Tests
相关产品
Description	Product Code	Applications	Pack Size
Human Seroblock	BUF070A	F	50 Test
Human Seroblock	BUF070B	F	200 Test
文献
1.monocytes.J Immunol. 167 (8): 4593-9.2.Voehringer, D.et al.(2002) Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectinlike receptor G1 (KLRG1).Blood. 100 (10): 3698-702.3.Piatier-Tonneau, D. (1997) CD4 workshop panel report.In: Leucocyte Typing VI: White Cell Differentiation Antigens: Proceedings of the Sixth International Workshop and Conference Held in Kobe, Japan, 10-14 November 1996. Garland Pub., 1998.4.Pentón-Rol, G.et al.(2011) C-Phycocyanin ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells.Int Immunopharmacol. 11 (1): 29-38.5.Wright, G.J.et al.(2001) The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.Immunology. 102 (2): 173-9.6.Zhang, Y.et al.(2013) Acceleratedin vivo.proliferation of memory phenotype CD4+T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism.PLoS Pathog. 9 (4): e1003310.7.Bughani, U.et al.(2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.PLoS One. 12 (7): e0180088.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Bio-Rad MCA1267F CD4抗体,Mouse anti Human CD4:FITC

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