Bio-Rad 1351303 DAPI,PUREBLU™ DAPI-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad 1351303 DAPI,PUREBLU™ DAPI

2024-10-23

PUREBLU™ DAPI

货号：1351303

规格：250 µg

价格：1003

产品类型：细胞染料

品牌：Bio-Rad

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

产品介绍
Use PureBlu DAPI Dye for routine nuclear staining in fluorescence microscopy and cell imaging applications. PureBlu DAPI Dye is a highly pure formulation of DAPI in a user-friendly format allowing easy preparation of the working solution, with no weighing step and only a single dilution after resuspension.DAPI (4',6-diamidino-2-phenylindole) is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. DAPI permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei (Figure 1).Fig. 1. DAPI binding dsDNA.When bound to dsDNA, PureBlu DAPI Dye has a maximum excitation wavelength in the ultraviolet range (359 nm), and the dye can be optimally detected in the blue channel with an emission maximum of 461 nm (Figure 2). The characteristic Stokes shift between excitation and emission wavelengths is fairly wide for DAPI, making this dye an optimal choice when good spectral separation is desired to reduce fluorescence interference, for example, in chromatin counterstaining for immunofluorescence microscopy.Fig. 2. DAPI excitation and emission spectra.

技术参数

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Bio-Rad 1351303 DAPI,PUREBLU™ DAPI

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