CD252 (OX40 Ligand) Monoclonal Antibody (RM134L), Alexa Fluor 488, eBioscience

货号：53-5905-82

规格：100 μg

市场价格：3068

产品类型：流式抗体

品牌：eBioscience

抗原：CD252

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：RM134L

荧光染料：Alexa Fluor 488

类型:	流式抗体	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor 488, eBioscience
浓度：	0.5 mg/mL	用法：	1.0 μg/test(Flow)

产品详细信息Description:

The RM134L monoclonal antibody reacts with mouse CD252 also known as OX-40 Ligand, a member of the TNF superfamily. OX-40L is induced on mouse splenic B cells stimulated with a combination of anti-IgM and anti-CD40. Neither resting nor activated mouse T cells express OX-40L. The interaction of OX-40 (CD134) with OX-40L has been implicated in T cell-dependent humoral response. RM134L inhibits the costimulatory activity of mouse OX-40L transfected cells for anti-CD3-stimulated T cellproliferation.Applications Reported: This RM134L antibody has been reported for use in flow cytometric analysis.Applications Tested: This RM134L antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

靶标信息

The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD252 (OX40 Ligand) Antibody (53-5905-82) in FlowC57BL/6 mouse splenocytes were stimulated for 72 hours with Mouse IgM Monoclonal Antibody, Functional Grade (Product # 16-5092-85) and CD40 Monoclonal Antibody, Functional Grade Purified (Product # 16-0401-85). Cells were then stained with 0.5 µg of Rat IgG2b kappa Isotype Control, Alexa Fluor 488 (Product # 53-4031-80) (blue histogram) or 0.5 µg of CD252 Monoclonal Antibody, Alexa Fluor 488 (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

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