Bio-Rad 1351101 READIDROP™ Propidium Iodide,READIDROP™ Propidium Iodide-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad 1351101 READIDROP™ Propidium Iodide,READIDROP™ Propidium Iodide

2024-10-23

READIDROP™ Propidium Iodide

货号：1351101

规格：3 x 3ml

价格：2338

产品类型：细胞增殖活性检测

品牌：Bio-Rad

物种：人/小鼠/非人灵长类/猪/恒河猴

宿主：驴

抗体亚型：IgG3

荧光染料：Alexa Fluor 488

产品介绍
Propidium Iodide (PI) is a fluorescent dye that binds to DNA and RNA in a stoichiometric ratio. PI is not membrane permeable and therefore requires the compromised membranes of dead cells to bind to DNA. Upon excitation with an appropriate light source, dead cells exhibit higher fluorescence intensity than live cells, thereby enabling clear discrimination of these populations by flow cytometry. Dead cells can then be excluded from further analysis and also sorted from cell populations of interest. The spectral properties (Excitation 535 nm/Emission 617 nm) enable use in both single color and multi color flow experiments. The convenient dropper bottle format removes the manual weighing, pipetting and dilution steps required by other commonly available PI products.
产品详情
Regulatory	For research purposes only
Guarantee	Guaranteed until date of expiry. Please see product label.
存储条件
Store at room temperature
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/250	1/500
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Instructions For Use Caution:Wear gloves, safety glasses/face protection, and protective clothing to avoid direct contact of ReadiDrop solutions with skin and eyes. Refer to MSDS for more information.1. Resuspend 1 x 106cells in 500 μl flow cytometry buffer (1x phosphate buffered saline with 3% BSA). The cell concentration is now 2 x 106/ml.2. Add one to two drop of ReadiDrop Propidium Iodide to the cell suspension.3. Vortex briefly.4. Incubate for 1-10 min at room temperature.Note:Adding one drop to a 500 μl cell suspension produces a final dye concentration of 1 μg/ml. This is typically sufficient to stain 1 x 106cells. If one drop is not sufficient for your cell type, adding a second drop may improve staining efficacy. When working with a larger volume of cells, scale ReadiDrop solution amount accordingly.5. Analyze cells by flow cytometry.- Detect PI signal in FL3 on Bio-Rad's S3e Cell Sorter. Otherwise, select the most appropriate FL channel on your particular instrument.
数据
Staining of a mixture of live and heat killed Jurkat cells with ReadiDrop Propidium Iodide for 1 min prior to analysis
文献
Weldrick, P.J.et al.(2020) Super‐Enhanced Removal of Fungal Biofilms by Protease‐Functionalized Amphotericin B NanocarriersAdv NanoBiomed Res. 10 Dec [Epub ahead of print].

技术参数

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Bio-Rad 1351101 READIDROP™ Propidium Iodide,READIDROP™ Propidium Iodide

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