Bio-Rad 1351102 READIDROP™ 7-AAD,READIDROP™ 7-AAD-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad 1351102 READIDROP™ 7-AAD,READIDROP™ 7-AAD

2024-10-23

READIDROP™ 7-AAD

货号：1351102

规格：3 x 3ml

价格：2338

产品类型：细胞增殖活性检测

品牌：Bio-Rad

物种：人/小鼠/非人灵长类/猪/恒河猴

宿主：驴

抗体亚型：IgG3

荧光染料：Alexa Fluor 488

产品介绍
7-aminoactinomycin (7-AAD) is a fluorescent dye that has a high affinity for GC-rich regions of double stranded DNA. 7-AAD is not membrane permeable and therefore requires the compromised membranes of dead cells to bind to DNA. Upon excitation with an appropriate light source, dead cells exhibit higher fluorescence intensity than live cells, thereby enabling clear discrimination of these populations by flow cytometry. Dead cells can then be excluded from further analysis and also sorted from cell populations of interest. The spectral properties (Excitation 546 nm/ Emission 647 nm) enable use in both single color and multi color flow experiments. The convenient dropper bottle format removes the manual weighing, pipetting and dilution steps required by other commonly available 7-AAD products.
产品详情
Regulatory	For research purposes only
Guarantee	Guaranteed until date of expiry. Please see product label.
存储条件
Store at room temperature
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/250	1/500
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Instructions For Use Caution:Wear gloves, safety glasses/face protection, and protective clothing to avoid direct contact of ReadiDrop solutions with skin and eyes.1. Resuspend 1 x 106cells in 500 μl flow cytometry buffer (1x phosphate buffered saline with 3% BSA). The cell concentration is now 2 x 106/ml.2. Add one to two drop of ReadiDrop 7-AAD to the cell suspension.3. Vortex briefly.4. Incubate for 1-10 min at room temperature.Note:Adding one drop to a 500 µl cell suspension produces a final dye concentration of 1 μg/ml. This is typically sufficient to stain 1 x 106cells. If one drop is not sufficient for your cell type, adding a second drop may improve staining efficacy. When working with a larger volume of cells, scale ReadiDrop solution amount accordingly.5. Analyze cells by flow cytometry.- Detect 7-AAD signal in FL3 on Bio-Rad's S3e Cell Sorter. Otherwise, select the most appropriate FL channel on your particular instrument.
数据
Staining of a mixture of live and heat killed Jurkat cells with ReadiDrop 7-AAD for 1 min prior to analysis
文献
Al Tanoury, Z.et al.(2020) Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineagein vitro.Development. 147 (12): dev187344.

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-44568.htm

特别声明：以上内容(如有图片亦包括在内)来源于授权厂家或网络，如有侵权，请联系删除，本平台不提供信息存储服务。

Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.

产品询价需求提交

Bio-Rad 1351102 READIDROP™ 7-AAD,READIDROP™ 7-AAD

相关推荐