Bio-Rad 12004165 ANTI-TUBULIN hFAB™ Rhodamine Antibody,ANTI-TUBULIN hFAB™ Rhodamine Antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad 12004165 ANTI-TUBULIN hFAB™ Rhodamine Antibody,ANTI-TUBULIN hFAB™ Rhodamine Antibody

2024-10-23

ANTI-TUBULIN hFAB™ Rhodamine Antibody

货号：12004165,12004166

规格：200 µl,40 µl

价格：8908,1845

产品类型：流式抗体

品牌：Bio-Rad

物种：人/小鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

产品介绍
hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDHantibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated withHuman Combinatorial Antibody Library (HuCAL®) Technology, providing exceptional specificity.These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc™ MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.Features and BenefitsSimple— No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivityVersatile— Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blottingUnique— HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other speciesDiverse— Antibodies have been tested to detect human or mouse housekeeping proteins
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Mouse	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	A monovalent human recombinant Fab selected from the HuCAL phage display library, expressed inE. coli.and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized
Reconstitution	Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at maximum speed on a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube.
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	>80% Sucrose<0.04% 2-Methyl-2H-Isothiazol-3-One<10% bovine serum albumin
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Rhodamine	530	580
Regulatory	For research purposes only
Guarantee	12 monthsfrom dateof despatch
存储条件
1 year at -20oC lyophilized; 6 months at +4oC after resuspension.This product is photosensitive and should be protected from lightDO NOT FREEZE the solubilized material
应用
Application Name	Verified	Min Dilution	Max Dilution
Western Blotting	√	1/1000	1/10000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
文献
1. Riera-Tur, I.et al.(2022) Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.Life Sci Alliance. 5(3):e202101185.2. Cotton, T.R.et al.(2021) Structural basis of K63-ubiquitin chain formation by the Gordon-Holmes syndrome RBR E3 ubiquitin ligase RNF216.Mol Cell S1097-2765 (21) 01067-4.3. Cejas, R.B.et al.(2022) Impact of DYRK1A Expression on TNNT2 Splicing and Daunorubicin Toxicity in Human iPSC-Derived Cardiomyocytes.Cardiovasc Toxicol. May 21 [Epub ahead of print].4. Wang, K.et al.(2022) Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions.Nucleic Acids Res. 50 (14): 7842-55.

技术参数

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Bio-Rad 12004165 ANTI-TUBULIN hFAB™ Rhodamine Antibody,ANTI-TUBULIN hFAB™ Rhodamine Antibody

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