Bio-Rad 12004163 ANTI-ACTIN hFAB™ Rhodamine Antibody,ANTI-ACTIN hFAB™ Rhodamine Antibody

2024-10-23

ANTI-ACTIN hFAB™ Rhodamine Antibody

货号:12004163,12004164

规格:200 µl,40 µl

价格:8908,1845

产品类型:流式抗体

品牌:Bio-Rad

物种:人/小鼠

宿主:其它

抗体亚型:IgG

荧光染料:其它

产品介绍
hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDHantibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated withHuman Combinatorial Antibody Library (HuCAL®) Technology, providing exceptional specificity.These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc MP Imaging System, and can be used in multiplex experiments with StarBright Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.Features and BenefitsSimple— No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivityVersatile— Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blottingUnique— HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other speciesDiverse— Antibodies have been tested to detect human or mouse housekeeping proteins
产品详情

Target Species

Human

Species Cross-ReactivityTarget SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Pack Size: 200 µl

A monovalent human recombinant Fab selected from the HuCAL phage display library, expressed inE. coli.and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized

Pack Size: 40 µl

A monovalent human recombinant Fab selected from the HuCAL phage display library, expressed inE. coli.and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized

Reconstitution

Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on

ice for atleast 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at maximum speed on a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube.

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

>80% Sucrose<0.04% 2-Methyl-2H-Isothiazol-3-One<10% bovine serum albumin

Immunogen

Normal human peripheral blood lymphocytes.

Max Ex/Em

FluorophoreExcitation Max (nm)Emission Max (nm)

Rhodamine

530580

Regulatory

For research purposes only

Guarantee

12 monthsfrom dateof despatch

存储条件
1 year at -20oC lyophilized; 6 months at +4oC after resuspension.This product is photosensitive and should be protected from lightDO NOT FREEZE the solubilized material
应用
Application NameVerifiedMin DilutionMax Dilution
Western Blotting1/10001/10000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Western Blotting
  • StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.

技术参数
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