eBioscience 11-0801-81,CD80 (B7-1) Monoclonal Antibody (16-10A1), FITC

2024-10-23

CD80 (B7-1) Monoclonal Antibody (16-10A1), FITC

货号:11-0801-81,11-0801-82,11-0801-85,11-0801-86

规格:50μg,100μg,500μg,1mg

价格:863,1698,2886,5234

产品类型:流式抗体

品牌:eBioscience

物种:小鼠

宿主:小鼠

抗体亚型:IgG

克隆号:16-10A1

荧光染料:FITC

类型:

单抗

同型对照:

Armenian Hamster IgG Isotype Control (eBio299Arm), FITC

浓度:

0.5 mg/mL

用法:

0.25 μg/test(Flow)
产品详细信息

Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: The 16-10A1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

数据

CD80 (B7-1) Antibody (11-0801-81) in Flow

Staining of unstimulated (left) or 3-day LPS-stimulated (right) C57BL/6 splenocytes with 0.125 µg of Anti-Mouse CD80 (B7-1) FITC. Total viable cells were used for analysis. Markers were set based on the autofluorescence sample.

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参考文献:

1.

Frontiers in immunology

FcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells.

"Published figure using CD80 (B7-1) monoclonal antibody (Product # 11-0801-82) in Flow Cytometry"

2.

Oncology reports

CD1d highly expressed on DCs reduces lung tumor burden by enhancing antitumor immunity.

"11-0801 was used in Flow cytometry/Cell sorting to investigate whether CD1d expression on dendritic cells is capable of enhancing anti-tumour immunity, particularly T-cell related immunity."

3.

Nature communications

T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells.

"Published figure using CD80 (B7-1) monoclonal antibody (Product # 11-0801-82) in Flow Cytometry"

4.

Theranostics

Mouse IP-10 Gene Delivered by Folate-modified Chitosan Nanoparticles and Dendritic/tumor Cells Fusion Vaccine Effectively Inhibit the Growth of Hepatocellular Carcinoma in Mice.

"11-0801 was used in Flow cytometry/Cell sorting to illustrate that the mIP-10 enhances the anti-tumor effect of DC/tumor cell fusion vaccine by alleviating the immunosuppressive tumor environment."

AuthorsHu Z,Chen J,Zhou S,Yang N,Duan S,Zhang Z,Su J,He J,Zhang Z,Lu X,Zhao Y

5.

Cancer medicine

MiR-486 promotes proliferation and suppresses apoptosis in myeloid cells by targeting Cebpa in vitro.

AuthorsJiang J,Gao Q,Gong Y,Huang L,Lin H,Zhou X,Liang X,Guo W

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