IL-12 p40 Human ELISA Kit ELISA试剂盒,IL-12 p40 Human ELISA Kit/人IL-12 p40 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-12 p40 Human ELISA Kit ELISA试剂盒,IL-12 p40 Human ELISA Kit/人IL-12 p40 ELISA试剂盒

2024-10-23

IL-12 p40 Human ELISA Kit/人IL-12 p40 ELISA试剂盒

货号：KAC1561

规格：96 tests

价格：7030

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	1.5 pg/mL
检测范围	15-1300 pg/mL
样本类型/体积	上清液 100 μL 血清 100μL 血浆 100μL
操作时间	1 hr 20 min
测试时间	4 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	6.7%
批内变异系数（CV）	4%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 孵育缓冲液 - HRP-共轭检测抗体 - 共轭稀释剂 - 洗涤液 - 显色剂 - 终止液 - 对照
储存温度	2-8℃
Protein name	IL-12 p40
Protein aliases	CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, IL12, subunit p40, interleukin 12, p40, interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), interleukin-12 beta chain, Interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD subunit, NK cell stimulatory factor chain 2, NKSF2
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	CLMF, CLMF2, IL-12B, IL12B, IMD28, IMD29, NKSF, NKSF2
Gene ID	(Human) 3593
Gene symbol	IL12B
UniProt ID	(Human) P29460

The Human Interleukin-12 p40(Hu IL-12 p40) ELISA quantitates Hu IL-12 p40 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-12 p40.人白介素12 p40（人 IL-12 p40）ELISA定量了人血清，血浆，缓冲液或细胞培养基中的人IL-12 p40。该检测试剂盒将只识别天然和重组的人IL-12 p40。Principle of the method 方法原理The Human IL-12 p40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-12 p40固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息IL12B (IL-12 p40) encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kDa cytokine receptor like subunit encoded by this gene, and a 35 kDa subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.IL12B（IL-12 p40）编码白介素12的一个亚基，白介素12是一种作用于T和自然杀伤细胞的细胞因子，具有广泛的生物学活性。白介素12是由该基因编码的40kDa细胞因子受体样亚基和IL12A编码的35kDa亚基组成的二硫键连接的异二聚体。这种细胞因子由活化的巨噬细胞表达，而巨噬细胞可作为Th1细胞发育的重要诱导剂。已经发现该细胞因子对于维持足够数量的记忆/效应Th1细胞以介导对细胞内病原体的长期保护很重要。在患有多发性硬化症（MS）的患者的中枢神经系统中观察到该基因的过表达，表明该细胞因子在疾病的发病机理中具有作用。据报道，该基因的启动子多态性与儿童特应性哮喘和非特应性哮喘的严重程度有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-12 p40 Human ELISA KitSchematic showing protein specificity for Human IL-12 p40 ELISA

技术参数

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IL-12 p40 Human ELISA Kit ELISA试剂盒,IL-12 p40 Human ELISA Kit/人IL-12 p40 ELISA试剂盒

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