Aggrecan (PG) Human ELISA Kit ELISA试剂盒,Aggrecan (PG) Human ELISA Kit/人Aggrecan (PG) ELISA试剂盒

2024-10-23

Aggrecan (PG) Human ELISA Kit/人Aggrecan (PG) ELISA试剂盒

货号:KAP1461

规格:96 tests

价格:7030

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.9 ng/mL

检测范围10-250 ng/mL

样本类型/体积

上清液 10 μL

血清 20μL

滑液 25 μL

操作时间

1 hr 20 min

测试时间

3 hr 15 min

Homogenous (no wash)

No

批间变异系数(CV)5.6%
批内变异系数(CV)2.1%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 Tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 孵育缓冲液

- HRP-共轭检测抗体

- 共轭稀释液

- 洗涤液

- 显色剂

- 终止液

- 对照

储存温度

2-8℃

Protein nameAggrecan
Protein aliasesAggrecan core protein, Aggrecan core protein 2, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan 1, Chondroitin sulfate proteoglycan core protein 1, CSPCP, large aggregating proteoglycan

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物HRP
Gene aliasesACAN, AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK
Gene ID(Human) 176
Gene symbolACAN
UniProt ID

(Human) P16112

The Human Aggrecan (PG) ELISA quantitates Hu Aggrecan (PG) in humansynovial fluid, serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Aggrecan (PG).人Aggrecan(PG)ELISA定量测定人滑液,血清,缓冲液或细胞培养基中的人Aggrecan(PG)。 该检测试剂盒将专门识别天然和重组的人Aggrecan(PG)。Principle of the method 方法原理The Human Aggrecan (PG) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second enzyme-conjugated (detector) antibody, binding to the target on a different epitope from the capture antibody. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人Aggrecan(PG)固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二种酶偶联(检测)抗体形成三明治,该抗体与捕获抗体在不同表位上的靶标结合。 经过孵育和洗涤步骤以去除未结合物质的微孔板后,添加底物溶液,该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.Aggrecan也称为软骨特异性蛋白聚糖核心蛋白(CSPCP)或硫酸软骨素蛋白聚糖1是由ACAN基因编码的蛋白。编码的蛋白质是软骨组织中细胞外基质的组成部分,并且可以承受软骨的压缩。该蛋白的人型长度为2316个氨基酸(约250 kDa),由于可变剪接,可以多种同工型表达。 Aggrecan展示了一种牙刷结构,其中硫酸软骨素和硫酸角质素链连接到扩展的蛋白核上。人们正在研究聚集蛋白聚糖的合成和降解在关节损伤,疾病和衰老过程中在软骨退化中的作用。它包含三个球间域(IGD),G1,G2和G3,它们与聚集,透明质酸结合,细胞粘附和软骨细胞凋亡有关。由于软骨聚集蛋白聚糖酶和MMP活性的切割,来自关节软骨的Aggrecan片段在人骨关节炎的所有阶段都释放到滑液中。抗体BC-3和BC-4可以分别用于检测Aggrecanase(ARGSVXXX)和MMP(XXXVDIPEN)的这些新表位。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

Aggrecan (PG) Human ELISA KitRepresentative data for Aggrecan/PG concentration in serum of normal subjects aged from 3 to 66 years.

技术参数
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