Osteocalcin Human ELISA Kit ELISA试剂盒,Osteocalcin Human ELISA Kit/人骨钙素ELISA试剂盒

2024-10-23

Osteocalcin Human ELISA Kit/人骨钙素ELISA试剂盒

货号:KAQ1381

规格:96 tests

价格:7227

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.08 ng/mL

检测范围1.56-75 ng/mL

样本类型/体积

血清 25 μL

血浆 25 μL

操作时间

1 hr 20 min

测试时间

2 hr 30 min

Homogenous (no wash)

No

批间变异系数(CV)4.6%
批内变异系数(CV)3.9%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 Tests

组份

- 预包被96孔板

- 标准品

- 标准稀释液

- 孵育缓冲液

- HRP-共轭检测抗体

- 洗涤液

- 显色剂

- 终止液

- 对照

储存温度

2-8℃

Protein nameOsteocalcin
Protein aliasesBGP, bone gamma-carboxyglutamate (gla) protein (osteocalcin), Bone Gla protein, Gamma-carboxyglutamic acid-containing protein, Osteocalcin

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物HRP
Gene aliasesBGLAP, BGP, OC, OCN
Gene ID(Human) 632
Gene symbolBGLAP
UniProt ID

(Human) P02818

The Human Osteocalcin ELISA quantitates Hu Osteocalcin in human serum and plasma,. The assay will exclusively recognize both natural and recombinant Hu Osteocalcin.人骨钙素ELISA可定量测定人血清和血浆中的骨钙素。 该检测试剂盒将只识别天然和重组的人骨钙素。Principle of the method 方法原理The Human Osteocalcin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人骨钙素固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.骨钙蛋白属于骨钙蛋白/基质Gla蛋白家族,占骨蛋白总量的1-2%。 它与磷灰石和钙牢固结合。 γ-羧基谷氨酸残基通过维生素K依赖的羧化作用形成。 这些残基对于钙的结合至关重要。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

Osteocalcin Human ELISA KitThe following data are for demonstration purpose only and cannot be used in place of data generated at the time of assay.

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