CD23 Human ELISA Kit ELISA试剂盒,CD23 Human ELISA Kit/人CD23 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

CD23 Human ELISA Kit ELISA试剂盒,CD23 Human ELISA Kit/人CD23 ELISA试剂盒

2024-10-23

CD23 Human ELISA Kit/人CD23 ELISA试剂盒

货号：KAS0251

规格：96 tests

价格：7030

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.15 U/mL
检测范围	1-20 U/mL
样本类型/体积	上清液 100 μL 血清 100μL 血浆 100μL
操作时间	1 hr 20 min
测试时间	2 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	4.2%
批内变异系数（CV）	3.2%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 标准稀释液 - 孵育缓冲液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 对照
储存温度	2-8℃
Protein name	CD23
Protein aliases	BLAST-2, C-type lectin domain family 4 member J, C-type lectin domain family 4, member J, CD23, CD23 antigen, Fc epsilon receptor II, Fc fragment of IgE, low affinity II, receptor for (CD23), Fc-epsilon-RII, Immunoglobulin E-binding factor, immunoglobulin epsilon-chain, Low affinity immunoglobulin epsilon Fc receptor, Low affinity immunoglobulin epsilon Fc receptor membrane-bound form, Low affinity immunoglobulin epsilon Fc receptor soluble form, Lymphocyte IgE receptor
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	BLAST-2, CD23, CD23A, CLEC4J, FCE2, FCER2, IGEBF
Gene ID	(Human) 2208
Gene symbol	FCER2
UniProt ID	(Human) P06734

The Human CD23 ELISA quantitates Hu CD23 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD23.人CD23 ELISA可定量人血清，血浆，缓冲液或细胞培养基中的人CD23。该检测试剂盒只识别天然和重组人CD23。Principle of the method 方法原理The Human CD23 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人CD23固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.CD23是一种45 kDa的糖蛋白，存在于新鲜分离的外周血和扁桃体B细胞的亚群中，并在EBV转化的B淋巴母细胞中强烈表达。 CD23分子与B细胞上发现的低亲和力IgE受体相同。在来自B细胞慢性淋巴细胞性白血病病例和某些中心粒性/中心性淋巴瘤病例的肿瘤细胞中已经检测到CD23的表达。 CD23存在于新鲜分离的外周血和扁桃体B细胞的亚群中，并在EBV转化的B淋巴母细胞上强烈表达。在功能上，CD23参与B细胞的生长和分化以及IgE的产生。此外，CD23具有可溶形式，其是有效的促有丝分裂因子。与CD23功能障碍有关的疾病包括慢性结膜炎和慢性淋巴细胞性白血病。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

CD23 Human ELISA KitRepresentative standard curve for Human soluble CD23 ELISA

技术参数

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CD23 Human ELISA Kit ELISA试剂盒,CD23 Human ELISA Kit/人CD23 ELISA试剂盒

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