IL-10 Human ELISA Kit EIISA试剂盒,IL-10 Human ELISA Kit/人IL-10 EIISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-10 Human ELISA Kit EIISA试剂盒,IL-10 Human ELISA Kit/人IL-10 EIISA试剂盒

2024-10-23

IL-10 Human ELISA Kit/人IL-10 EIISA试剂盒

货号：KHC0101,KHC0102,KHC0101C

规格：96 Tests,2X96 Tests,5X96 Tests

价格：7303,10140,23831

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<1 pg/mL
检测范围	7.8-500 pg/mL
样本类型/体积	上清液 50 μL 血清 50μL 血浆 50μL
操作时间	1 hr 20 min
测试时间	5hr
Homogenous (no wash)	No
批间变异系数（CV）	3.7%
批内变异系数（CV）	3.5%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	5X96 Tests
组份	- 预包被96孔板 - 标准品 - 标准稀释液 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 辣根过氧化物酶稀释液 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-10
Protein aliases	CSIF, Cytokine synthesis inhibitory factor, IL-10, Interleukin-10, T-cell growth inhibitory factor
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	CSIF, GVHDS, IL-10, IL10, IL10A, TGIF
Gene ID	(Human) 3586
Gene symbol	IL10
UniProt ID	(Human) P22301

The Human Interlukin 10 (IL-10) ELISA quantitates Hu IL-10 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-10.人白介素10（IL-10）ELISA定量了人血清，血浆，缓冲液或细胞培养基中的人IL-10。该检测试剂盒将只识别天然和重组的人IL-10。Principle of the method 方法原理The Human IL-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-10固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.白介素10（IL-10，CSIF）是一种主要由巨噬细胞和Th2细胞产生的抗炎细胞因子。 IL-10是一个160个氨基酸残基的18.6 kDa蛋白，与爱泼斯坦-巴尔病毒蛋白（BCRFI）具有80％以上的序列同源性。已报道的IL-10生物学活性包括抑制巨噬细胞介导的细胞因子合成，抑制迟发型超敏反应以及刺激Th2细胞反应，从而导致抗体产生增加。 IL-10通过抑制巨噬细胞和调节性T细胞（包括IFN-γ，TNF-α，IL-2和IL-3，IL-4和GM-CSF）产生的促炎细胞因子发挥功能。已知IL-10抑制抗原呈递细胞上的抗原呈递，增强B细胞存活，增殖和抗体产生。 IL-10可以阻断NF-κB的活性，并参与JAK-STAT信号通路的调节。虽然人IL-10可以作用于鼠细胞，但是鼠IL-10不能作用于人细胞。与细胞视黄醇结合蛋白II启动子（CRBPII-RXRE）的类视黄醇X受体（RXR）响应元件结合。 IL-10抑制视黄酸响应元件的9-顺式视黄酸依赖性RXRα转录激活。小鼠的基因敲除研究表明，这种细胞因子在肠道中是必需的免疫调节剂。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-10 Human ELISA KitRepresentative data using Human serum samples on Human IL-10 ELISA, and compared to a competitor ELISA. Data shows a correlation factor of R2 = 0.9669.

技术参数

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IL-10 Human ELISA Kit EIISA试剂盒,IL-10 Human ELISA Kit/人IL-10 EIISA试剂盒

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