EGF Human ELISA Kit ELISA试剂盒,EGF Human ELISA Kit/人EGF ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

EGF Human ELISA Kit ELISA试剂盒,EGF Human ELISA Kit/人EGF ELISA试剂盒

2024-10-23

EGF Human ELISA Kit/人EGF ELISA试剂盒

货号：KHG0061,KHG0062

规格：96 Tests,2X96 Tests

价格：7301,11232

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<1 pg/mL
检测范围	3.9-250 pg/mL
样本类型/体积	上清液 100 μL 血清 100μL 血浆 100μL 尿液 10μL
操作时间	1 hr 20 min
测试时间	4 hr
Homogenous (no wash)	No
批间变异系数（CV）	5.3%
批内变异系数（CV）	4.4%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 标准稀释液 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 辣根过氧化物酶稀释液 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	EGF
Protein aliases	beta-urogastrone, EGF, Urogastrone, Epidermal growth factor, Pro-epidermal growth factor
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	EGF, HOMG4, URG
Gene ID	(Human) 1950
Gene symbol	EGF
UniProt ID	(Human) P01133

The Human Epidermal Growth Factor (EGF) ELISA quantitates Hu EGF in human serum, plasma, buffered solution, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu EGF.人表皮生长因子（EGF）ELISA对人血清，血浆，缓冲液，尿液或细胞培养基中的人EGF进行定量。该检测试剂盒将专门识别天然和重组人EGF。Principle of the method 方法原理The Human EGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人EGF固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.EGF（表皮生长因子）通过与170 kDa的蛋白激酶EGF受体结合而发挥作用。 EGFR的激活可响应毒性环境刺激或EGF与受体的结合而启动多种细胞途径，EGFR与其他家族成员形成同型或异型二聚体。每个二聚体受体复合物通过募集含有效应蛋白的不同Src同源性2（SH2）来启动不同的信号传导途径。 EGF在肾脏，大脑，前列腺和唾液腺中广泛表达。 EGF充当有力的促有丝分裂因子，磷酸化受体募集衔接子蛋白（如GRB2）来激活复杂的下游信号传导级联反应。 EGF激活至少4个主要的下游信号传导级联，包括RAS-RAF-MEK-ERK，PI3激酶-AKT，PLCgamma-PKC和STAT模块。研究表明，该蛋白在激活NF-κB信号级联反应中也可能起重要作用。 EGGF基因的缺陷是4型低镁血症的病因，失调与某些癌症的生长和进展有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

EGF Human ELISA KitRepresentative data using human serum samples on Human EGF ELISA, and compared to a competitor ELISA. Data shows a correlation factor of R2 = 0.9985.

技术参数

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EGF Human ELISA Kit ELISA试剂盒,EGF Human ELISA Kit/人EGF ELISA试剂盒

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