Prostaglandin E2 Human ELISA Kit/人前列腺素E2 ELISA试剂盒

货号：KHL1701

规格：96 tests

价格：5660

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

检测范围	31.3-4000 pg/mL
样本类型/体积	上清液 100 μL 血浆 100μL 尿液 100μL
操作时间	1 hr 20 min
测试时间	3 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	11.7%
批内变异系数（CV）	6%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预涂96孔板 - 标准品 - Tris缓冲剂 - 碱性磷酸酶示踪剂 - 白三烯B4抗血清 - DEA缓冲液 - 洗涤液 - pNPP片 - 胶板盖
Protein name	Prostaglandin E2
Protein aliases	C20H32O5, PGE2
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	AP

The Human Prostaglandin E2 ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (ELISA). This assay is designed to detect and quantify the level of human prostaglandin E2 in biological samples.人前列腺素E2 ELISA试剂盒是一种竞争性的酶联免疫吸附测定（ELISA）。 该检测试剂盒将检测和定量生物样品中人前列腺素E2的水平。Principle of the method 方法原理This assay is based on the competition between Prostaglandin E 2 (PGE2) and a PGE2 alkaline phosphatase tracer for a limited amount of PGE2 specific monoclonal antibody. Because of the competition between PGE2 in the sample and PGE2 tracer for the PGE2 antibody, the signal obtained with the assay will be inversely proportional to the amount of PGE 2 in each sample.该测定法基于前列腺素E 2（PGE2）和PGE2碱性磷酸酶示踪剂对有限的PGE2特异性单克隆抗体的竞争。 由于样品中的PGE2和PGE2示踪剂之间存在PGE2抗体之间的竞争，因此通过分析获得的信号将与每个样品中PGE 2的量成反比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Prostoglandin E2 (PGE2) is a potent lipid mediator produced by metabolism of arachidonic acid via the cyclooxygenase (COX) pathway. Following cellular stimulation, arachidonic acid is hydrolyzed from phospholipids stores by one of a family of phospholipase A2 enzymes and converted to the unstable endoperoxide PGH2 by either COX 1 or COX 2. PGH2 is isomerized to PGE2 by one of 3 distinct enzymes microsomal PGES 1 (mPGES 1), mPGES 2, or a cytosolic enzyme, cPGES. PGE2 is active in a number of physiological systems and settings including inflammation, immune regulation, generation of fever, pain perception, protection of the gastric muscosa, fertility and parturition, as well as sodium and water retention. The effects of PGE2 are transduced by 4 subtypes of G protein coupled receptors designated EP1, EP2, EP3, EP4, with Kd values for PGE2 ranging from 1 10 nM. PGE2 is rapidly metabolized in vivo by the prostaglandin 15 dehydrogenase pathway (15 hydroxy PGDH) to the inactive metabolite 13,14 dihydro 15 keto PGE2. The half life of PGE2 in the circulatory system is ~30 seconds and normal plasma levels are 3 12 pg/mL.前列腺素 E2（PGE2）是一种有效的脂质介体，由花生四烯酸经由环氧合酶（COX）途径代谢而产生。在细胞刺激后，花生四烯酸被磷脂酶A2酶家族中的一种从磷脂库中水解出来，并被COX 1或COX 2转化为不稳定的过氧化物PGH2。PGH2被微粒体PGES 1的3种不同酶之一异构化为PGE2（ mPGES 1），mPGES 2或胞质酶cPGES。 PGE2在许多生理系统和环境中均具有活性，包括炎症，免疫调节，发烧，疼痛感，保护胃粘膜，生育力和分娩以及钠和水water留。 PGE2的作用可通过G蛋白偶联受体的4个亚型（分别称为EP1，EP2，EP3，EP4）进行转导，PGE2的Kd值范围为1 10 nM。 PGE2在体内通过前列腺素15脱氢酶途径（15羟基PGDH）迅速代谢为非活性代谢物13,14二氢15酮PGE2。 PGE2在循环系统中的半衰期约为30秒，正常血浆水平为3 12 pg / mL。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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