Cytokeratin 5 Monoclonal Antibody (EP1601Y)

货号：MA5-14473

规格：1 mL

价格：11833

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：A synthetic peptide corresponding to residues on t

物种：人/小鼠

宿主：兔

抗体亚型：IgG

克隆号：EP1601Y

荧光染料：其它

类型:	单抗	同型对照：	IgG
浓度：		用法：	5.0 µg/mL(ICC);5 µg/mL(IF);1:50(IHC);1:100(IHC(P));1:500(WB)

产品详细信息MA5-14473 targets Cytokeratin 5 in IHC (P) applications and shows reactivity with Human samples.The MA5-14473 immunogen is a synthetic peptide corresponding to residues on the C-term of human CK5.靶标信息Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

Cytokeratin 5 Antibody (MA5-14473) in IFImmunofluorescence analysis of Cytokeratin 5 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 5 Monoclonal Antibody (Product # MA5-14473) at 5µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification. Cytokeratin 5 Antibody (MA5-14473) in IHCImmunohistochemistry analysis of Cytokeratin 5 was performed on cryosections of human skin tissue. Tissues were blocked in 10% normal goat serum in 1X PBS containing 0.1% Triton X-100 (1X PBS-T) for 1 hour at room temperature (RT). The tissues were labeled with a Cytokeratin 5 monoclonal antibody (clone EP1601Y, red,Product # MA5-14473) diluted 1:50 in 3% normal goat serum in 1X PBS-T for 1 hour at RT, followed by detection with a Goat anti-Rabbit IgG (H+L), Alexa Fluor 568 secondary antibody (Product # A-11036) diluted 1:2000 in 3% normal goat serum in 1X PBS-T for 1 hour at RT. Nuclei (blue) were stained with DAPI, included in ProLong Gold Anti-Fade Mountant (Product # P-36931). Images were taken on an inverted microscope at 20X magnification. Data courtesy of Dr. Jiyoon Lee at Indiana University School of Medicine. Cytokeratin 5 Antibody (MA5-14473) in IHCFormalin-fixed, paraffin-embedded human mesothelioma stained with Keratin 5 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining. Cytokeratin 5 Antibody (MA5-14473) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), MCF7 (Lane 2) and tissue extracts (30 µg lysate) of Mouse skin. The blot was probed with Anti-Cytokeratin 5 Monoclonal Antibody (Product # MA5-14473, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 62 kDa band corresponding to Cytokeratin 5 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1.Developmental cellFgf10-Hippo Epithelial-Mesenchymal Crosstalk Maintains and Recruits Lung Basal Stem Cells.2.BMC medical genomicsMolecular subtypes of urothelial carcinoma are defined by specific gene regulatory systems.3.Naturep63(+)Krt5(+) distal airway stem cells are essential for lung regeneration.4.The Journal of pathologyA novel blueprint for 'top down' differentiation defines the cervical squamocolumnar junction during development, reproductive life, and neoplasia.5.International journal of dermatologyAn immunohistochemical study of the origin of the solid strand in syringoma, using carcinoembryonic antigen, epithelial membrane antigen, and cytokeratin 5.

技术参数

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