Cytokeratin 5 Monoclonal Antibody (XM26)/KRT5单抗

货号：MA5-12596

规格：500 µL

价格：4880

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：Recombinant protein corresponding to C-terminal 10

物种：人/小鼠

宿主：小鼠

抗体亚型：其它

克隆号：XM26

荧光染料：其它

类型:	单抗	同型对照：	IgG1, kappa
浓度：		用法：	1:250(ICC);1:250(IF);1:50(IHC); 1:50(IHC(P));1:250(WB)

产品详细信息MA5-12596 targets Cytokeratin 5 in IHC (P) applications and shows reactivity with Human samples.The MA5-12596 immunogen is recombinant protein corresponding to C-terminal 103 aa of cytokeratin 5.靶标信息Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

Cytokeratin 5 Antibody (MA5-12596) in IFImmunofluorescence analysis of Cytokeratin 5 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 5 (XM26) Mouse Monoclonal Antibody (Product # MA5-12596) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification. Cytokeratin 5 Antibody (MA5-12596) in IHCFormalin-fixed, paraffin-embedded human mesothelioma stained with Keratin 5 antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells. Cytokeratin 5 Antibody (MA5-12596) in WBWestern blot analysis was performed on Whole cell extract (30 µg lysate) of A431. The blot was probed with Anti-Cytokeratin 5 Mouse Monoclonal Antibody (Product # MA5-12596, 1:250 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A ~60 kDa band corresponding to Cytokeratin 5 was observed in the cell line tested. Known quantity of protein sample was electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1.Molecular cancer research : MCRChemokine Signaling Facilitates Early-Stage Breast Cancer Survival and Invasion through Fibroblast-Dependent Mechanisms.2.Translational oncologyContinuous Delivery of Neutralizing Antibodies Elevate CCL2 Levels in Mice Bearing MCF10CA1d Breast Tumor Xenografts.3.HIV medicineHIV nucleoside reverse transcriptase inhibitors efavirenz and tenofovir change the growth and differentiation of primary gingival epithelium.4.HistopathologyA systematic analysis of commonly used antibodies in cancer diagnostics.5.Clinics (Sao Paulo, Brazil)Immunohistochemical profile of high-grade ductal carcinoma in situ of the breast.

技术参数

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