CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), eBioscience™

货号：14-9400-82,14-9400-80

规格：100 µg,25 µg

价格：3099,1244

产品类型：流式抗体

品牌：eBioscience

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

克隆号：ME20.4

荧光染料：其它

类型:	单抗	同型对照：	IgG1, kappa
浓度：	0.5 mg/mL	用法：	0.25 µg/test(Flow);Assay-Dependent(ICC);Assay-Dependent(IF);Assay-Dependent(WB);5 µg/mL(IHC(P))

产品详细信息Description: The monoclonal antibody ME20.4 recognizes CD271, a 75 kDa type I transmembrane protein that belongs to the low-affinity neurotrophin receptor and tumor necrosis factor receptor superfamily. CD271 is expressed by neurons, Schwann cells, melanocytes, mesenchymal stem/stromal cells, neural crest stem cells, astrocytes, oligodendrocytes, and a subset of lymphoid cells including follicular dendritic cells. CD271 binds to the ligands NGF, BDNF, NTF3, and NTF4. CD271 contains a death domain which induces apoptosis and regulates cell growth and proliferation through interactions with TRAF2, TRAF4, TRAF6, PTPN13, and RANBP9.The ME20.4 monoclonal antibody recognizes human, non-human primate, mouse, rat, sheep, rabbit, cat, dog, and pig CD271.Applications Reported: This ME20.4 antibody has been reported for use in flow cytometric analysis, western blotting, microscopy, immunohistochemical staining, and immunocytochemistry.Applications Tested: This ME20.4 antibody has been tested by flow cytometric analysis of SK-N-SH cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This ME20.4 antibody has also been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Neurotrophic factors control the survival, differentiation and maintenance of neurons in the peripheral and central nervous systems, and of other neural crest-derived cell types. Developing sympathetic neurons are absolutely dependent upon nerve growth factor (NGF) during the period of target competition in vivo. Antibodies reacting specifically with NGFR p75 are useful tools in the detection and characterization of NGFR p75, to enhance our understanding of a wide range of phenomena in the development, plasticity and repair of the nervous system.

数据

CD271 (NGF Receptor) Antibody (14-9400-82) in IHC (P)Immunohistochemistry of formalin-fixed paraffin embedded human prostate tissue stained with 5 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 5 µg/mL of Anti-CD271 (NGF Receptor) Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin. CD271 (NGF Receptor) Antibody (14-9400-82)Nucleic acids research 2018 -Figure 4. piggyBac /Ad-GFP transduces multiple cell types. Tissue from each region of the pig lung (as depicted in Figure 1B ) was sectioned, counterstained with DAPI, and imaged. ( A and B ) piggyBac /Ad-GFP transduces submucosal glands (SMGs) throughout the trachea. ( B and C ) Tissues stained with acetyl-tubulin (red) show that piggyBac /Ad-GFP transduces ciliated (c) and non-ciliated (nc) cells at the airway surface. ( D - F ) piggyBac /Ad-GFP co-localizes with CK5 positive basal cells (red) within submucosal glands ( n = 6). Scale bars for A = 250 mum; B-F = 500 mum. ( G and H ) piggyBac /Ad-GFP transduces basal cells at the airway surface. Arrows indicate GFP positive cells that co-localize with red (G) NGFR or (H) CK14 immunostaining. Images acquired at 63x magnification ( n = 6)

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参考文献：

1.Nucleic acids researchWidespread airway distribution and short-term phenotypic correction of cystic fibrosis pigs following aerosol delivery of piggyBac/adenovirus.

技术参数

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