IL-18 Mouse ELISA Kit/小鼠IL-8 ELISA试剂盒
货号：KMC0181
规格：96 tests
价格：10143
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 25 pg/mL |
| 检测范围 | 31.2-2000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 100 μL 血浆 100 μL |
操作时间 | 1 hr 20 min |
测试时间 | 2 hr 30 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | 5% |
| 批内变异系数（CV） | 3.8% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 标准稀释液 - HRP共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 |
储存温度 | 2-8℃ |
| Protein name | IL-18 |
| Protein aliases | IFN-gamma-inducing factor, IL-1 gamma, IL-18, Interferon gamma-inducing factor, interferon-gamma-inducing factor, Interleukin-1 gamma, Interleukin-18 |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | HRP |
| Gene aliases | Igif, Il-18, Il18 |
| Gene ID | (Mouse) 16173 |
| Gene symbol | Il18 |
| UniProt ID | (Mouse) P70380 |
The Mouse Interleukin 18 (Ms IL-18) ELISA quantitates Ms IL-18 in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-18.小鼠白介素18（小鼠IL-18）ELISA定量检测小鼠血清，血浆，缓冲液或细胞培养基中的小鼠IL-18。 该检测试剂盒将专门识别天然和重组小鼠IL-18。Principle of the method 方法原理The Mouse IL-18 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠IL-18固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.该基因编码的蛋白质是促炎细胞因子。 该细胞因子可以诱导T细胞的IFN-γ产生。 该细胞因子和IL12的组合已显示抑制IL4依赖性IgE和IgG1的产生，并增强B细胞IgG2a的产生。 IL-18结合蛋白可以与该细胞因子特异性相互作用，从而对其生物活性产生负调控。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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