IL-8 Porcine ELISA Kit ELISA试剂盒,IL-8 Porcine ELISA Kit/猪IL-8 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-8 Porcine ELISA Kit ELISA试剂盒,IL-8 Porcine ELISA Kit/猪IL-8 ELISA试剂盒

2024-10-23

IL-8 Porcine ELISA Kit/猪IL-8 ELISA试剂盒

货号：KSC0081,KSC0082

规格：96 Tests,2X96 Tests

价格：7227,11232

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<10 pg/mL
检测范围	31.2-2000 pg/mL
样本类型/体积	上清液 100 μL 血清 50 μL
操作时间	1 hr 20 min
测试时间	4 hr
Homogenous (no wash)	No
批间变异系数（CV）	8.6%
批内变异系数（CV）	7.1%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	2X96 Tests
组份	- 预包被96孔板 - 标准品 - 标准稀释液 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶（HRP） - HRP稀释液 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-8
Protein aliases	Alveolar macrophage chemotactic factor I, alveolar macrophage-derived chemotactic factor-I, AMCF-I, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, IL-8, Interleukin-8
物种（Tested）	猪
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	AMCF-I, CXCL8, IL8
Gene ID	(Pig) 396880
Gene symbol	IL8
UniProt ID	(Pig) P26894

The Porcine Interleukin 8 (Por IL-8) ELISA quantitates Por IL-8 in porcine serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Por IL-8.猪白介素8（猪IL-8）ELISA定量检测猪血清，缓冲溶液或细胞培养基中的猪 IL-8。该检测试剂盒将只识别天然和重组的猪IL-8。Principle of the method 方法原理The Porcine IL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.猪IL-8固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.白介素8（IL-8，CXCL8）是一种72个氨基酸的促炎因子，属于趋化因子的CXC亚家族，并与细胞表面受体IL-8RA和IL-8RB结合。 IL-8用作化学吸引剂和有效的血管生成因子。 IL-8的表达和分泌可通过多种细胞（包括巨噬细胞和内皮细胞）中的多种炎症刺激来诱导。在内皮细胞中，IL-8存在于称为Weibel-Palade体的储存小泡中。首先从骨肉瘤细胞中分离出来的IL-8含有ELR-基序（N端Glu-Leu-Arg氨基酸序列），并通过CXCR1和CXCR2受体发出信号。 IL-8的先前命名法包括嗜中性粒细胞激活蛋白1（NAP-1），粒细胞趋化蛋白1（GCP-1），单核细胞衍生的嗜中性粒细胞激活肽（MONAP）和蛋白3-10C。 IL-8和CXC趋化因子基因家族的其他十个成员在映射到染色体4q的区域中形成趋化因子基因簇。癌症研究表明IL-8在肿瘤的血管生成和生长中起作用，据信IL-8在毛细支气管炎（一种由病毒感染引起的常见呼吸道疾病）的发病机理中起作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-8 Porcine ELISA KitRepresentative standard curve for Swine IL-8 ELISA

技术参数

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IL-8 Porcine ELISA Kit ELISA试剂盒,IL-8 Porcine ELISA Kit/猪IL-8 ELISA试剂盒

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