Adiponectin Rat ELISA Kit ELISA试剂盒,Adiponectin Rat ELISA Kit/大鼠脂联素ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Adiponectin Rat ELISA Kit ELISA试剂盒,Adiponectin Rat ELISA Kit/大鼠脂联素ELISA试剂盒

2024-10-23

Adiponectin Rat ELISA Kit/大鼠脂联素ELISA试剂盒

货号：KRP0041

规格：96 tests

价格：0

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：大鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	50 pg/mL
检测范围	0.375-24 ng/mL
样本类型/体积	上清液 100 μL 血清 10 μL 血浆 10 μL
操作时间	1 hr 20 min
测试时间	3 hr 20 min
Homogenous (no wash)	No
批间变异系数（CV）	4.9%
批内变异系数（CV）	4.8%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 标准稀释液 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 -辣根过氧化物酶稀释剂 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	Adiponectin
Protein aliases	adipocyte complement related protein of 30 kDa, adiponectin
物种（Tested）	大鼠
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	Acdc, Acrp30
Gene ID	(Rat) 246253
Gene symbol	Adipoq

The Rat Adiponectin ELISA quantitates Rat Adiponectin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rat Adiponectin.大鼠脂联素ELISA定量测定人血清，血浆，缓冲液或细胞培养基中的大鼠脂联素。该检测试剂盒将只识别天然和重组大鼠脂联素。Principle of the method 方法原理The Rat Adiponectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.大鼠脂联素固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.脂联素是由脂肪细胞分泌的一种蛋白质激素，可调节脂质和葡萄糖的代谢。脂联素在各种生理过程中都有作用，包括体内稳态和肥胖。脂联素水平降低与肥胖者以及胰岛素抵抗和高胰岛素血症（2型糖尿病）有关。在小鼠中脂联素已显示改善胰岛素信号传导，从而改善胰岛素抵抗。通过激活AMP激酶和PPAR-α，脂联素可以增加骨骼肌中的脂肪酸转运，氧化和耗散，从而导致肌内脂质水平降低，从而改善胰岛素信号传导。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

Adiponectin Rat ELISA KitRepresentative standard curve for Rat Adiponectin ELISA

技术参数

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Adiponectin Rat ELISA Kit ELISA试剂盒,Adiponectin Rat ELISA Kit/大鼠脂联素ELISA试剂盒

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