PAI1 Human ELISA Kit ELISA试剂盒,PAI1 Human ELISA Kit/人PAI1 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

PAI1 Human ELISA Kit ELISA试剂盒,PAI1 Human ELISA Kit/人PAI1 ELISA试剂盒

2024-10-23

PAI1 Human ELISA Kit/人PAI1 ELISA试剂盒

货号：BMS2033,BMS2033TEN

规格：96 Tests,10X96 Tests

价格：6120,34832

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	29.0 pg/mL
检测范围	78-5,000 pg/mL
样本类型/体积	血清 10 μL 血浆 10 μL 上清液 10 μL
操作时间	1 hr 20 min
测试时间	3 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	5%
批内变异系数（CV）	4.7%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	PAI1
Protein aliases	Endothelial plasminogen activator inhibitor, PAI, PAI-1, Serpin E1, Plasminogen activator inhibitor 1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, serpin peptidase inhibitor
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	PAI, PAI-1, PAI1, PLANH1, SERPINE1
Gene ID	(Human) 5054
Gene symbol	SERPINE1
UniProt ID	(Human) P05121

The Human Plasminogen Activator Inhibitor-1 (Hu PAI-1) ELISA quantitates Hu PAI-1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant HuPAI-1.人纤溶酶原激活物抑制剂1（人PAI-1）ELISA定量分析了人类血清，血浆，缓冲溶液或细胞培养基中的人PAI-1。该检测试剂盒将专门识别天然和重组人PAI-1。Principle of the method 方法原理The Human PAI-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人PAI-1固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息PAI1 (plasminogen activator inhibitor 1) belongs to serine protease inhibitor superfamily, and is the principal inhibitor of tissue-type and urokinase-type plasminogen activators (tPA and uPA). Platelets are the main source of the circulating PAI11, but it is synthesized and secreted by many tissue and cell types, including fibroblasts, smooth muscle cells, endothelial cells, hepatocytes, and inflammatory cells. Expression of PAI11 can be regulated at the transcriptional level by many factors including growth factors, cytokines, hormones, inflammatory factors, glucose or lipid metabolites, vascular tone regulating factors, chemicals, and other environmental or physical factors. PAI1 is present at increased levels in various disease states, and has been linked to an increased occurrence of thrombosis in obesity, thrombophilia and the metabolic syndrome. Defects in PAI-1 are characterized by abnormal bleeding. PAI1 mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator, and may promote neuronal survival. Other defects in PAI1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency). Alternatively spliced transcript variants encoding different isoforms of PAI1 have been found.PAI1（纤溶酶原激活物抑制剂1）属于丝氨酸蛋白酶抑制剂超家族，是组织型和尿激酶型纤溶酶原激活物（tPA和uPA）的主要抑制剂。血小板是循环中PAI11的主要来源，但它是由许多组织和细胞类型合成和分泌的，包括成纤维细胞，平滑肌细胞，内皮细胞，肝细胞和炎性细胞。可以通过许多因素在转录水平上调节PAI11的表达，这些因素包括生长因子，细胞因子，激素，炎性因子，葡萄糖或脂质代谢产物，血管紧张度调节因子，化学物质以及其他环境或物理因素。 PAI1在各种疾病状态下的含量都较高，并且与肥胖症，血栓形成和代谢综合征中血栓形成的发生率增加相关。 PAI-1的缺陷是异常出血。 PAI1通过抑制纤溶酶原激活剂的活性来介导纤维蛋白溶解的抑制，并可能促进神经元存活。 PAI1中的其他缺陷是纤溶酶原激活物抑制剂1缺乏症（PAI-1缺乏症）的原因。已经发现编码PAI1的不同同工型的剪接的转录物变体For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

PAI1 Human ELISA KitRepresentative standard curve for Human PAI-1 ELISA

技术参数

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