Caspase 9 Human ELISA Kit ELISA试剂盒,Caspase 9 Human ELISA Kit/人半光天冬酶9 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Caspase 9 Human ELISA Kit ELISA试剂盒,Caspase 9 Human ELISA Kit/人半光天冬酶9 ELISA试剂盒

2024-10-23

Caspase 9 Human ELISA Kit/人半光天冬酶9 ELISA试剂盒

货号：BMS2025,BMS2025TEN

规格：96 Tests,10X96 Tests

价格：6120,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.4 ng/mL
检测范围	1.6-100 ng/mL
样本类型/体积	血清 50 μL 上清液 50 μL 细胞裂解液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	9%
批内变异系数（CV）	6.6%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 标准稀释液 - 测定稀释浓缩液 - 检测抗体 - 抗-兔HRP共轭物 - 裂解液 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	Caspase 9
Protein aliases	APAF-3, apoptotic protease activating factor 3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, caspase 9, apoptosis-related cysteine peptidase, Caspase-9, Caspase-9 subunit p10, Caspase-9 subunit p35, ICE-LAP6, ICE-like apoptotic protease 6, protein phosphatase 1, regulatory subunit 56
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	APAF-3, APAF3, CASP9, ICE-LAP6, MCH6, PPP1R56
Gene ID	(Human) 842
Gene symbol	CASP9
UniProt ID	(Human) P55211

The Human Caspase 9 ELISA quantitates Hu Caspase 9 in human serum, cell lysate or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Caspase 9.人半胱天冬酶9 ELISA定量人类血清，细胞裂解液或细胞培养基中的人半胱天冬酶9。该检测试剂盒将专门识别天然和重组人半光天冬酶9。Principle of the method 方法原理The Human Caspase 9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人半光天冬酶9固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer’s Disease (AD).半胱天冬酶9（ICE样凋亡蛋白酶6，ICE LAP6，凋亡蛋白酶Mch6，凋亡蛋白酶激活因子3，Apaf3）是肽酶家族C14的成员，其包含CARD结构域。半胱氨酸蛋白酶，表达为无活性的前体，通过特定底物的蛋白水解来介导凋亡。半胱天冬酶9作为异四聚体具有活性，存在于细胞质中，并在活化后易位至线粒体。半胱天冬酶9参与负责凋亡执行的半胱天冬酶激活级联，并裂解/激活半胱天冬酶3和半胱天冬酶6。此外，半胱天冬酶9参与负责凋亡执行的半胱天冬酶激活级联。半胱天冬酶-9与Apaf-1的结合导致蛋白酶的活化，然后裂解并活化半胱天冬酶-3。半胱天冬酶9以ABL1 / c-Abl依赖性方式促进DNA损伤诱导的凋亡，并通过蛋白水解方式切割聚（ADP-核糖）聚合酶（PARP）。半胱天冬酶9在多种人体组织中表达。已鉴定出大鼠半胱天冬酶-9的同工型，其中全长半胱天冬酶-9的C末端被替代的肽序列取代。半胱天冬酶9中的突变影响大脑发育。在小鼠中，胱天蛋白酶9不足会导致大脑发育异常和神经元发育。在人类中，功能异常的半胱天冬酶9表达因组织而异。低水平的半胱天冬酶9可能在癌症发展和神经退行性疾病（例如阿尔茨海默氏病（AD））中起作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

Caspase 9 Human ELISA KitRepresentative standard curve for Human Caspase-9 ELISA

技术参数

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Caspase 9 Human ELISA Kit ELISA试剂盒,Caspase 9 Human ELISA Kit/人半光天冬酶9 ELISA试剂盒

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