IL-13 Mouse ELISA Kit/小鼠IL-13 ELISA试剂盒
货号：BMS6015,BMS6015TWO,BMS6015FIVE,BMS6015TEN
规格：96 Tests,2X96 Tests,5X96 Tests,10X96 Tests
价格：6120,10105,17562,35475
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 2.8 pg/mL |
| 检测范围 | 7.8-500 pg/mL |
样本类型/体积 | 血清 50 μL 血浆 50 μL 上清液 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 3 hr 10 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | 5% |
| 批内变异系数（CV） | 4.6% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 x 96 tests |
组份 | - 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | IL-13 |
| Protein aliases | IL-13, T-cell activation protein P600, Interleukin-13 |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Il-13, Il13 |
| Gene ID | (Mouse) 16163 |
| Gene symbol | Il13 |
| UniProt ID | (Mouse) P20109 |
| The Mouse Interleukin-13 (Ms IL-13) ELISA quantitates Ms IL-13 in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-13.小鼠白介素13（小鼠IL-13）ELISA定量检测小鼠血清，血浆，缓冲液或细胞培养基中的小鼠IL-13。 该检测试剂盒将专门识别天然和重组小鼠IL-13。Principle of the method 方法原理The Mouse IL-13 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠IL-13固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.IL-13主要由活化的Th2细胞产生的免疫调节细胞因子，参与调节炎症和免疫反应。 IL-13下调巨噬细胞活性，从而抑制促炎性细胞因子和趋化因子的产生，并通过独立于IgE和嗜酸性粒细胞的机制参与过敏原诱发的哮喘。 IL-13还调节B细胞成熟和分化的几个阶段，上调CD23和MHC II类表达，并促进B细胞的IgE同种型转换。 IL-13在结构上与IL-3，IL-4，IL-5和GM-CSF非常相似。 编码IL-13，IL3，IL5，IL4和CSF2的基因在5q染色体上形成细胞因子基因簇，其中IL-13基因特别接近IL4。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 数据： |
IL-13 Mouse ELISA KitRepresentative standard curve for Mouse IL-13 ELISA |
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