GM-CSF Human ELISA Kit ELISA试剂盒,GM-CSF Human ELISA Kit/人GM-CSF ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

GM-CSF Human ELISA Kit ELISA试剂盒,GM-CSF Human ELISA Kit/人GM-CSF ELISA试剂盒

2024-10-23

GM-CSF Human ELISA Kit/人GM-CSF ELISA试剂盒

货号：BMS283,BMS283TEN

规格：96 Tests,10X96 Tests

价格：6120,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	2.9 pg/mL
检测范围	7.8-500 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 羊水 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	4.4%
批内变异系数（CV）	6%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	GM-CSF
Protein aliases	colony stimulating factor 2 (granulocyte-macrophage), Colony-stimulating factor, CSF, GM-CSF, Molgramostin, Sargramostim, granulocyte macrophage-colony stimulating factor, Granulocyte-macrophage colony-stimulating factor
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	CSF2, GMCSF
Gene ID	(Human) 1437
Gene symbol	CSF2
UniProt ID	(Human) P04141

The Human Granulocyte Macrophage Colony Stimulating Factor (Hu GM-CSF) ELISA quantitates Hu GM-CSF in human serum, plasma, amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GM-CSF.人粒细胞巨噬细胞集落刺激因子（人GM-CSF）ELISA定量了人血清，血浆，羊水或细胞培养基中的人GM-CSF。该检测试剂盒将专门识别天然和重组人GM-CSF。Principle of the method 方法原理The Human GM-CSF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人GM-CSF固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息GM-CSF (Granulocyte-Macrophage colony-stimulating factor) is a 14.6kDa hematopoietic growth factor that exists in glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The active form of the GM-CSF protein is found extracellularly as a homodimer and the GM-CSF gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. The ability of recombinant GM-CSF to increase hematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation. Recent studies have investigated GM-CSF in inflammatory and autoimmune diseases such as arthritis, arthritis related interstitial lung disease, nephritis, and psoriasis. In the CNS, GM-CSF depletion or neutralization has been studied in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS).GM-CSF（粒细胞-巨噬细胞集落刺激因子）是一种14.6kDa的造血生长因子，以糖基化和非糖基化的生物活性形式存在，并刺激粒细胞，巨噬细胞，早期巨核细胞和嗜酸性粒细胞祖细胞的发育。 GM-CSF蛋白的活性形式在细胞外被发现为同型二聚体，并且GM-CSF基因已定位在5q31号染色体区域的相关基因簇中，该基因与5q综合征和5q综合征的间质性缺失有关。急性骨髓性白血病。簇中的其他基因包括编码白介素4、5和13的基因。重组GM-CSF增加造血细胞恢复能力已成为骨髓移植后患者治疗的重点领域。最近的研究已经研究了GM-CSF在炎性和自身免疫性疾病中，例如关节炎，与关节炎相关的间质性肺病，肾炎和牛皮癣。在中枢神经系统中，已经在实验性自身免疫性脑脊髓炎（EAE）和多发性硬化症（MS）中研究了GM-CSF的消耗或中和。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

GM-CSF Human ELISA KitRepresentative standard curve for Human GM-CSF ELISA

技术参数

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