IL-21 Mouse ELISA Kit ELISA试剂盒,IL-21 Mouse ELISA Kit/小鼠IL-21 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-21 Mouse ELISA Kit ELISA试剂盒,IL-21 Mouse ELISA Kit/小鼠IL-21 ELISA试剂盒

2024-10-23

IL-21 Mouse ELISA Kit/小鼠IL-21 ELISA试剂盒

货号：BMS6021,BMS6021TEN

规格：96 Tests,10X96 Tests

价格：6120,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：小鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	5.0 pg/mL
检测范围	16.1-1,000 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	5.1%
批内变异系数（CV）	5.2%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-21
Protein aliases	IL-21, Interleukin-21
物种（Tested）	小鼠
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	IL-21, Il21
Gene ID	(Mouse) 60505
Gene symbol	Il21
UniProt ID	(Mouse) Q9ES17

The Mouse Interleukin-21 (Ms IL-21) ELISA quantitates Ms IL-21 in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-21.小鼠白介素-21（小鼠IL-21）ELISA定量检测小鼠血清，血浆，缓冲液或细胞培养基中的小鼠IL-21。该检测试剂盒将专门识别天然和重组小鼠IL-21。Principle of the method 方法原理The Mouse IL-21 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠IL-21固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.IL-21是一种17 kDa的免疫调节细胞因子，主要由自然杀伤细胞（NKT），T辅助细胞（Th）17和T滤泡辅助细胞（TFH）细胞产生。在TFH细胞中，IL-21表达导致通过IL-21受体（IL-21R）和STAT3的自分泌信号传导，从而导致Bcl6进行额外的转录激活。与Th1细胞的IFNγ和Th2细胞的IL-4一样，IL-21对于TFH细胞发育和效应子功能至关重要。 IL-21在依赖T细胞的B细胞分化为浆细胞和记忆细胞，刺激IgG产生和诱导天然B细胞凋亡信号传导中发挥作用。在Th17细胞中，IL-21的表达和通过STAT3，IRF4和RORγt引起的自分泌反馈导致IL-23R上调，从而为Th17细胞做好准备，使其通过炎性细胞因子IL-23进行成熟和维持。 IL-21在上调IRF4和RORγt的同时，还介导Foxp3的下调。 IL-21缺陷小鼠由于无法上调与Th17相关的分子而受到化学治疗，免受发展性结肠炎的侵害。相比之下，化学诱导的结肠炎小鼠模型中存在IL-21的升高水平。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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IL-21 Mouse ELISA Kit ELISA试剂盒,IL-21 Mouse ELISA Kit/小鼠IL-21 ELISA试剂盒

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