CCL2 (MCP-1) Monoclonal Antibody (2H5), Biotin, eBioscience/CCL2（MCP-1）单克隆抗体（2H5），生物素，eBioscience

货号：13-7096-81,13-7096-85

规格：50 gµ,500 gµ

价格：0,4115

产品类型：流式抗体

品牌：eBioscience

物种：人/小鼠/大鼠

宿主：其它

抗体亚型：IgG

克隆号：2H5

荧光染料：Biotin

类型:	单抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience
浓度：	0.5 mg/mL	用法：	Assay Dependent(Flow)；0.5-2 µg/mL（ELISA）

产品详细信息Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.Applications Reported: The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization. (Please use Functional Grade purified 2H5, cat. 16-7096, in functional assays.).Applications Tested: Mouse MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse MCP-1 in combination with the affinity purified 4E2/MCP (14-7091) antibody for capture and recombinant mouse MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.Human MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the affinity purified 5D3-F7 (14-7099) antibody for capture and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.The Functional Grade purified 2H5 antibody has been tested for neutralization of MCP-1 bioactivity.The fluorochrome-conjugated 2H5 antibody has been tested for intracellular staining and flow cytometric analysis of mouse, rat, and human cells and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

数据

CCL2 (MCP-1) Antibody (13-7096-81) in FlowStaining of mouse thiogylcolate induced peritoneal exudate cells (PECs) stimulated with LPS in the presence of Brefeldin A for 24 hours. with Anti-mouse F4/80 FITC (Product # 11-4801-82) followed by intracellular staining with Rat IgG2a kappa Isotype Control PE (Product # 12-4321-82) (left) or Anti-Mouse CCL2 PE. CCL2 (MCP-1) Antibody (13-7096-81)Molecular medicine reports 2015 -Figure 5 Migration of fibroblasts, HUVECs and UC-MSCs in response to UC-CM. (A) A total of 5x10 4 cells were collected and allowed to migrate. Lane 1, UC-CM; lane 2, DMEM; lanes 3-6, in the presence or absence of anti-SDF-1 (20 mu g/ml), anti-MCP-1 (20 mu g/ml) or anti-HGF (20 mu g/ml), respectively. Results are from a representative experiment and are expressed as the mean number of migrated cells in three random fields, scale bar=200 mu m. Cells that crossed the matrigel membrane were stained with crystal violet (magnification, x40). (B) Graphical presentation of the quantified data, presented as the number of migrated cells and expressed as the mean +- standard error of the mean. HUVECs, human umbilical vein endothelial cells; UC-MSCs, umbilical cord mesenchymal stem cells; UC-CM, UC-MSCs conditioned medium; DMEM, Dulbecco and apos;s modified Eagle and apos;s medium; SDF-1, stromal cell-derived factor 1; MCP-1, monocyte chemotactic protein 1; HGF, hepatocyte growth factor.

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流式抗体

参考文献：

1.Clinical biochemistryDevelopment and validation of an immunosensor for monocyte chemotactic protein 1 using a silicon photonic microring resonator biosensing platform.2.Arteriosclerosis, thrombosis, and vascular biologyOxidized low-density lipoprotein induces long-term proinflammatory cytokine production and foam cell formation via epigenetic reprogramming of monocytes.3.PloS oneThe liver X receptor agonist GW3965 improves recovery from mild repetitive traumatic brain injury in mice partly through apolipoprotein E.4.Molecular medicine reportsConditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis.5.PloS oneCCR2 defines a distinct population of NK cells and mediates their migration during influenza virus infection in mice.

技术参数

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