HSP27 Human ELISA Kit/人HSP27 ELISA试剂盒
货号：BMS2086,BMS2086TWO,BMS2086FIVE,BMS2086TEN
规格：96 Tests,2X96 Tests,5X96 Tests,10X96 Tests
价格：6120,8846,18643,35475
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 10.9 pg/mL |
| 检测范围 | 15.6-1,000 pg/mL |
样本类型/体积 | 血清 10 μL 血浆 10 μL 上清液 10 μL 细胞裂解液 10 μL |
操作时间 | 1 hr 20 min |
测试时间 | 3 hr 30 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | 4.3% |
| 批内变异系数（CV） | 5.8% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 x 96 tests |
组份 | - 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 对照 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | HSP27 |
| Protein aliases | 28 kDa heat shock protein, epididymis secretory protein Li 102, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, Heat shock protein beta-1, HSP 27, HspB1, SRP27, Stress-responsive protein 27 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | CMT2F, HEL-S-102, HMN2B, HS.76067, Hsp25, HSP27, HSP28, HSPB1, SRP27 |
| Gene ID | (Human) 3315 |
| Gene symbol | HSPB1 |
| UniProt ID | (Human) P04792 |
The Human Heat Shock Protein 27(Hu HSP27) ELISA quantitates Hu HSP27 in human serum, plasma, cell lysates, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HSP27.人热激蛋白27（人HSP27）ELISA定量分析了人类血清，血浆，细胞裂解液或细胞培养基中的人HSP27。 该检测试剂盒将专门识别天然和重组人HSP27。Principle of the method 方法原理The Human HSP27 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人HSP27固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.为了应对环境中的不利变化，来自许多生物的细胞会增加一类蛋白质的表达，这种蛋白质称为热激蛋白或应激蛋白。 HSPB1（热激蛋白β-1或HSP27）是一种小的热激蛋白，它起分子伴侣的作用，将变性蛋白保持在折叠状态。 在抗压力和肌动蛋白组织中发挥作用。 通过其分子伴侣活性，HSP27调节了许多生物过程，包括神经丝蛋白的磷酸化和轴突运输。 该基因的突变可导致Charcot-Marie-Tooth病2F和Neuronopathy远端遗传性运动2B。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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