FceR1 Human ELISA Kit ELISA试剂盒,FceR1 Human ELISA Kit/人FceR1 ELISA试剂盒

2024-10-23

FceR1 Human ELISA Kit/人FceR1 ELISA试剂盒

货号:BMS2101,BMS2101TEN

规格:96 Tests,10X96 Tests

价格:0,0

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.068 ng/mL

检测范围0.078-5.00 ng/mL

样本类型/体积

血清 20 μL

血浆 20 μL

上清液 20 μL

操作时间

1 hr 20 min

测试时间

4 hr 30 min

Homogenous (no wash)

No

批间变异系数(CV)6.8%
批内变异系数(CV)6.1%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- HRP-共轭检测抗体

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameFceR1 alpha
Protein aliasesFc epsilon receptor Ia, Fc epsilon RI alpha-chain, Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide, Fc IgE receptor, Fc-epsilon RI-alpha, FcERI, IgE Fc receptor subunit alpha, high affinity immunoglobulin epsilon receptor alpha-subunit, High affinity immunoglobulin epsilon receptor subunit alpha, immunoglobulin E receptor, high-affinity, of mast cells

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物HRP
Gene aliasesFCE1A, FCER1A, FcERI
Gene ID(Human) 2205
Gene symbolFCER1A
UniProt ID(Human) P12319
The Human Fc Epsilon Receptor 1 (Hu FcεR1) ELISA quantitates Hu FcεR1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FcεR1.人Fc Epsilon受体1(人FcεR1)ELISA定量检测人血清,血浆,缓冲液或细胞培养基中的人FcεR1。 该检测试剂盒将只识别天然和重组人FcεR1。Principle of the method 方法原理The Human FcεR1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人FcεR1固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE. Fc epsilonR1 alpha is a tetrameric complex consisting of one alpha, one ß and two additional subunits. The latter two are required for signal transduction activity. The Fc epsilonR1 complex plays an important role in triggering allergic responses, and the immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released. The IgE receptor couples allergen and mast cells to initiate the inflammatory responses that are characteristic of disorders such as hay fever and asthma. The release of histamine and proteases leads to the synthesis of prostaglandins and leukotrienes-potent effectors of the hypersensitivity response.FceR1(Fc epsilonR1 alpha)是IgE高亲和力受体的亚基。 Fc epsilonR1α是由一个α,一个ß和两个其他亚基组成的四聚体复合物。 后两者是信号转导活性所必需的。 Fc epsilonR1复合物在触发过敏反应中起重要作用,而免疫球蛋白ε受体(IgE受体)是过敏反应的引发剂。 当两个或多个高亲和力IgE受体通过结合变应原的IgE分子聚集在一起时,会释放引起过敏症状的介质,例如组胺。 IgE受体将变应原和肥大细胞耦合,以启动炎症反应,这些疾病是花粉症和哮喘等疾病的特征。 组胺和蛋白酶的释放导致超敏反应的前列腺素和白三烯有效效应子的合成。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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