IL-29 Human ELISA Kit ELISA试剂盒,IL-29 Human ELISA Kit/人IL-29 ELISA试剂盒

2024-10-23

IL-29 Human ELISA Kit/人IL-29 ELISA试剂盒

货号:BMS2049,BMS2049TEN

规格:96 Tests,10X96 Tests

价格:6120,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

2.0 pg/mL

检测范围15.6-1,000 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

3 hr 30 min

Homogenous (no wash)

No

批间变异系数(CV)7.1%
批内变异系数(CV)7.6%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameIL-29
Protein aliasesCytokine Zcyto21, IFN-lambda-1, Interleukin-29, IL-29, Interferon lambda-1, interferon, lambda 1, interferon-lambda-1, interleukin 29 (interferon, lambda 1)

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesIFNL1, IL-29, IL29, ZCYTO21
Gene ID(Human) 282618
Gene symbolIFNL1
UniProt ID(Human) Q8IU54
The Human Interleukin-29 (Hu IL-29) ELISA quantitates Hu IL-29 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-29.人白介素29(人IL-29)ELISA定量了人血清,血浆,缓冲液或细胞培养基中的人IL-29。 该检测试剂盒将只识别天然和重组的人IL-29。Principle of the method 方法原理The Human IL-29 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-29固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息IFN-lambdas constitute a novel family of interferons that mediate the induction of anti-viral protection in a wide variety of cells. There are three members in this family, namely lambda1, lambda2, and lambda3, which are also known as IL-29, IL-28A, and IL-28B, respectively. Similar to type I IFNs, IFN-lambdas activate an intracellular signaling to promote gene expression. IFN-lambdas mediate their anti-viral protection through a class II cytokine receptor complex composed of two essential receptor proteins, CRF2-12/IFN-lambdaR1, which is unique to IFN-lambdas, and CFR2-4/IL-10R2, which is shared with IL-10, IL-22, and IL-26 receptors. The IFN-lambdas, IL-28 and IL-29, have recently been reported to prime dendritic cells to induce proliferation of Foxp3-bearing regulatory T cells. DCs that differentiate in response to IFN-lambdas express high levels of class I and II MHC gene products, but low levels of costimulatory molecules. Moreover, these cells can specifically induce IL-2-dependent proliferation of CD4+CD25+FOXP3+ T cells with contact-dependent suppressive activity.干扰素-λ构成了干扰素的新家族,其介导了多种细胞中抗病毒保护的诱导。该家族中有3个成员,分别是lambda1,lambda2和lambda3,分别称为IL-29,IL-28A和IL-28B。类似于I型IFN,IFN-λ激活细胞内信号传导以促进基因表达。 IFN-λ通过II类细胞因子受体复合物介导其抗病毒保护作用,该复合物由两种必需的受体蛋白CRF2-12 /IFN-λR1(对于IFN-λ而言是独特的)和CFR2-4 / IL-10R2组成,后者是与IL-10,IL-22和IL-26受体共有。最近已经报道了IFN-λ,IL-28和IL-29引发树突状细胞,从而诱导带有Foxp3的调节性T细胞的增殖。响应IFN-λ分化的DC表达高水平的I和II类MHC基因产物,但表达低水平的共刺激分子。而且,这些细胞可以特异性地诱导具有接触依赖性抑制活性的CD4 + CD25 + FOXP3 + T细胞的IL-2依赖性增殖。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

IL-29 Human ELISA KitRepresentative standard curve for Human IL-29 (Interferon lambda) ELISA

技术参数
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