GRO alpha (CXCL1) Human ELISA Kit ELISA试剂盒,GRO alpha (CXCL1) Human ELISA Kit/人GRO α (CXCL1) ELISA试剂盒

2024-10-23

GRO alpha (CXCL1) Human ELISA Kit/人GRO α (CXCL1) ELISA试剂盒

货号:BMS2122

规格:96 Tests

价格:6120

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

7.6 pg/mL

检测范围16-1,000 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

3 hr 30 min

Homogenous (no wash)

No

批间变异系数(CV)5.1%
批内变异系数(CV)6.7%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameCXCL1 (GRO alpha)
Protein aliasesC-X-C motif chemokine 1, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha), fibroblast secretory protein, GRO-alpha(1-73), GRO-alpha(4-73), GRO-alpha(5-73), GRO-alpha(6-73), GRO1 oncogene (melanoma growth stimulating activity, GRO1 oncogene (melanoma growth-stimulating activity), Growth-regulated alpha protein, melanoma growth stimulating activity, alpha, Melanoma growth stimulatory activity, melanoma growth stimulatory activity alpha, MGSA, MGSA alpha, NAP-3, Neutrophil-activating protein 3

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesCXCL1, FSP, GRO, GRO1, GROA, MGSA, MGSA-a, NAP-3, SCYB1
Gene ID(Human) 2919
Gene symbolCXCL1
UniProt ID(Human) P09341
The Human GRO alpha (CXCL1) (Hu GROα) ELISA quantitates Hu GROα in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GROα.人GRO α(CXCL1)(人GROα)ELISA定量检测人血清,血浆,缓冲液或细胞培养基中的人GROα。 该检测试剂盒将专门识别天然和重组人GROα。Principle of the method 方法原理The Human GROα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人GROα固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.CXCL1,CXCL2和CXCL3,也分别称为GRO α,GRO β(MIP-2 α)和GRO γ(MIP-2 β),是趋化因子CXC亚家族的成员。 成熟的CXCL1 / 2/3蛋白与B型IL-8受体具有高亲和力,并且是有效的嗜中性粒细胞引诱剂和激活剂。 在体外,加工后的形式GRO-α(4-73),GRO-α(5-73)和GRO-α(6-73)显示出30倍高的趋化活性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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