L-Selectin (CD62L) (Soluble) Human ELISA Kit ELISA试剂盒,L-Selectin (CD62L) (Soluble) Human ELISA Kit/人选择素L(CD62L) (可溶) ELISA试剂盒

2024-10-23

L-Selectin (CD62L) (Soluble) Human ELISA Kit/人选择素L(CD62L) (可溶) ELISA试剂盒

货号:BMS206,BMS206TEN

规格:96 Tests,10X96 Tests

价格:6120,34832

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.198 ng/mL

检测范围0.4-25.0 ng/mL

样本类型/体积

血清 10 μL

血浆 10 μL

上清液 10 μL

操作时间

1 hr 20 min

测试时间

2 hr 10 min

Homogenous (no wash)

No

批间变异系数(CV)4.2%
批内变异系数(CV)3.7%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- HRP-共轭检测抗体

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameL-Selectin (CD62L)
Protein aliasesCD62 antigen-like family member L, CD62L, gp90-MEL, L-selectin, LAM-1, LECAM1, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, lymphocyte adhesion molecule 1, pln homing receptor, TQ1

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物HRP
Gene aliasesCD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, SELL, TQ1
Gene ID(Human) 6402
Gene symbolSELL
UniProt ID(Human) P14151
The Human L-Selectin (CD62L) ELISA quantitates Hu L-Selectin in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu L-Selectin.人L-选择素(CD62L)ELISA定量分析人血清,血浆或细胞培养基中的人L-选择素。 该检测试剂盒将专门识别天然和重组人L-选择素。Principle of the method 方法原理The Human L-Selectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人L-选择蛋白固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.人CD62L是粘附素选择素家族的74-95 kDa糖蛋白成员。 L-选择蛋白由氨基末端C型凝集素结合结构域,表皮生长因子样结构域,两个与补体结合蛋白中发现的同源短重复序列(SCR)同源,短间隔区,跨膜区和 胞质短区。 人CD62L(L-选择素)在所有类型的白细胞上组成性表达,包括淋巴细胞(大量记忆T细胞除外),单核细胞和多形核细胞。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

L-Selectin (CD62L) (Soluble) Human ELISA KitRepresentative standard curve for Human soluble l-Selectin ELISA

技术参数
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