TGF beta-1 Mouse ELISA Kit ELISA试剂盒,TGF beta-1 Mouse ELISA Kit/小鼠TGF β-1 ELISA试剂盒

2024-10-23

TGF beta-1 Mouse ELISA Kit/小鼠TGF β-1 ELISA试剂盒

货号:BMS608-4,BMS608-4TWO,BMS608-4FIVE,BMS608-4TEN

规格:96 Tests,2X96 Tests,5X96 Tests,10X96 Tests

价格:6120,8030,17562,34832

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:小鼠

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

7.8 pg/mL

检测范围31.25-2,000 pg/mL

样本类型/体积

血清 20 μL

血浆 20 μL

上清液 20 μL

操作时间

1 hr 20 min

测试时间

4 hr

Homogenous (no wash)

No

批间变异系数(CV)5.8%
批内变异系数(CV)7.9%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- HRP-共轭检测抗体

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameTGF beta-1
Protein aliasesLAP, Latency-associated peptide, regulatory protein, TGF-beta 1, TGF-beta-1, Transforming growth factor beta-1, Transforming growth factor beta-1 proprotein, transforming growth factor-beta 1

物种(Tested)

小鼠

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesTGF-beta1, Tgfb, Tgfb-1, Tgfb1, TGFbeta1
Gene ID(Mouse) 21803
Gene symbolTgfb1
UniProt ID(Mouse) P04202
The Mouse Transforming Growth Factor beta 1 (Ms TGFβ1) ELISA quantitates Ms TGFβ1in Mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Mouse TGFβ1.小鼠转化生长因子β 1(小鼠TGFβ1)ELISA定量检测小鼠血清,血浆,缓冲液或细胞培养基中的小鼠TGFβ1。 该检测试剂盒将专门识别天然和重组的小鼠TGFβ1。Principle of the method 方法原理The Mouse TGFβ1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠TGFβ1固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.TGF β-1(TGFB1,转化生长因子β 1)是细胞因子转化生长因子β超家族的多肽成员,普遍存在于组织中。转化生长因子(TGF)-b作为潜在复合物以其前结构域形式存储在细胞外基质中。 TGFβ-1的激活需要aV整联蛋白与前结构域中的RGD序列结合,并在该结构域上施加作用力,该作用域由潜在的TGF-b结合蛋白保持在细胞外基质中。潜在形式是TGFβ-1,TGF-β前体的氨基末端部分,称为TGF-LAP(TGF-潜伏期相关肽)和特定结合蛋白(称为LTBP)的复合物。 TGF beta-1有助于调节许多细胞类型的增殖,分化,粘附,迁移。许多细胞具有TGFβ受体,并且该蛋白可以正负调节许多其他生长因子。 TGFβ-1裂解为潜伏期相关肽和成熟的TGFβ-1肽,并以由TGFB1同二聚体,LAP同二聚体和潜在TGFB1结合蛋白组成的潜伏形式存在,或在活性形式由TGFβ-1同型二聚体组成。成熟的肽还可以与其他TGFβ家族成员形成异二聚体。 TGFβ-1的基因在肿瘤细胞中经常被上调,该基因的突变导致Camurati-Engelmann病和囊性纤维化。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

TGF beta-1 Mouse ELISA KitRepresentative standard curve for Mouse TGF beta1 ELISA

技术参数
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