MMP9 Human ELISA Kit ELISA试剂盒,MMP9 Human ELISA Kit/人MMP9 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

MMP9 Human ELISA Kit ELISA试剂盒,MMP9 Human ELISA Kit/人MMP9 ELISA试剂盒

2024-10-23

MMP9 Human ELISA Kit/人MMP9 ELISA试剂盒

货号：BMS2016-2,BMS2016-2TEN

规格：96 Tests,10X96 Tests

价格：6120,34832

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.05 ng/mL
检测范围	0.23-15.0 ng/mL
样本类型/体积	血清 10 μL 血浆 10 μL 上清液 10 μL
操作时间	1 hr 20 min
测试时间	3 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	10.2%
批内变异系数（CV）	7.3%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 对照 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	MMP-9
Protein aliases	67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, macrophage gelatinase, matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase), matrix metalloproteinase 9 (gelatinase B, Matrix metalloproteinase-9, MMP-9, type V collagenase
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	CLG4B, GELB, MANDP2, MMP-9, MMP9
Gene ID	(Human) 4318
Gene symbol	MMP9
UniProt ID	(Human) P14780

The Human Matrix Metalloproteinase 9 (Hu MMP-9) ELISA quantitates Hu MMP-9 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusivy recognize both natural and recombinant Hu MMP-9.人基质金属蛋白酶9（人MMP-9）ELISA定量了人血清，血浆，缓冲液或细胞培养基中的人MMP-9。该检测试剂盒将只识别天然和重组人MMP-9。Principle of the method 方法原理The Human MMP-9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人MMP-9固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.MMP9（基质金属肽酶9，GELB，CLG4B）是基质金属蛋白酶，是降解细胞外基质蛋白的锌和钙依赖性内肽酶家族。 MMP9被分泌为92kDa的酶原，MMP9的裂解导致活性酶的分子量为82kDa。 MMP9具有由三个纤连蛋白II型单元组成的明胶结合结构域，包含锌结合位点的催化结构域，富含脯氨酸的V型胶原同源结构域和血红素样结构域。 MMP9由单核细胞，巨噬细胞，嗜中性粒细胞，角质形成细胞，成纤维细胞，破骨细胞和内皮细胞产生，并参与炎症反应，组织重塑，伤口愈合，肿瘤生长和转移。 MMP9由骨髓来源的细胞提供，并有助于皮肤癌变。此外，MMP9降解IV型和V型胶原蛋白。在恒河猴中的研究表明，该酶参与了IL-8诱导的骨髓造血祖细胞的动员，而鼠类研究表明，其在肿瘤相关的组织重塑中起作用。研究表明，MMP9升高与特发性房颤和主动脉瘤的进展有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

MMP9 Human ELISA KitRepresentative standard curve for Human MMP-9 ELISA

技术参数

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MMP9 Human ELISA Kit ELISA试剂盒,MMP9 Human ELISA Kit/人MMP9 ELISA试剂盒

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