MICB Human ELISA Kit ELISA试剂盒,MICB Human ELISA Kit/人MICB ELISA试剂盒

2024-10-23

MICB Human ELISA Kit/人MICB ELISA试剂盒

货号:BMS2303

规格:96 Tests

价格:5967

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

1.24 pg/mL

检测范围3-200 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

4 hr

Homogenous (no wash)

No

批间变异系数(CV)7.1%
批内变异系数(CV)4.6%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 tests

组份

- 预包被96孔板

- 标准品(2瓶)

- 标准稀释液

- 生物素化检测抗体

-Streptavidin-HRP

- HRP稀释液

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameMICB
Protein aliasesMHC class I chain-related protein B, MHC class I mic-B antigen, MHC class I polypeptide-related sequence B, MIC-B, MHC class I-like molecule PERB11.2-IMX, MICB, stress inducible class I homolog

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesMICB, PERB11.2
Gene ID(Human) 4277
Gene symbolMICB
UniProt ID(Human) Q29980
The Human MICB ELISA quantitates Hu MICB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MICB.人MICB ELISA可以定量人血清,血浆或细胞培养基中的人MICB。 该检测试剂盒将专门识别天然和重组人MICB。Principle of the method 方法原理The Human MICB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人MICB固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,该第二抗体与与捕获抗体不同的表位上的靶标结合。 已将结合酶掺入测定中。 经过孵育和洗涤步骤以去除未结合物质的微孔板后,添加底物溶液,该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息MICB encodes the highly polymorphic MHC (HLA) class I chain-related gene B. The protein product is expressed on the cell surface. It is thought that MICB functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. MICB is broadly recognized by NK cells and T cells with NKG2D receptor on their surface. The complex NKG2D-MICB results in MICB expressing cytolytic T cells and NK cells against epithelial tumor cells.MICB编码高度多态性的MHC(HLA)I类链相关基因B。蛋白质产物在细胞表面表达。 认为MICB起应激诱导抗原的作用,其被肠上皮γ-δT细胞广泛识别。 MICB被表面具有NKG2D受体的NK细胞和T细胞广泛识别。 复杂的NKG2D-MICB导致针对上皮肿瘤细胞的表达MICB的溶细胞性T细胞和NK细胞。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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