NGAL Human ELISA Kit ELISA试剂盒,NGAL Human ELISA Kit/人NGAL ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

NGAL Human ELISA Kit ELISA试剂盒,NGAL Human ELISA Kit/人NGAL ELISA试剂盒

2024-10-23

NGAL Human ELISA Kit/人NGAL ELISA试剂盒

货号：BMS2202

规格：96 tests

价格：6120

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	6.5 pg/mL
检测范围	7.81-500 pg/mL
样本类型/体积	血清 10 μL 血浆 10 μL 上清液 10 μL
操作时间	1 hr 20 min
测试时间	3 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	5.8%
批内变异系数（CV）	4%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	NGAL
Protein aliases	25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, migration-stimulating factor inhibitor, Neutrophil gelatinase-associated lipocalin, NGAL, Oncogene 24p3, p25, Siderocalin, siderocalin LCN2
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	24p3, HNL, LCN2, MSFI, NGAL, p25
Gene ID	(Human) 3934
Gene symbol	LCN2
UniProt ID	(Human) P80188

The Human Neutrophil Gelatinase-Associated Lipocalin (Hu NGAL) ELISA quantitates Hu NGAL in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NGAL.人嗜中性粒细胞明胶酶相关的脂蛋白（人NGAL）ELISA定量了人类血清，血浆，缓冲溶液或细胞培养基中的人NGAL。该检测试剂盒将专门识别天然和重组人NGAL。Principle of the method 方法原理The Human NGAL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人NGAL固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.NGAL（中性粒细胞明胶酶相关的脂环蛋白，脂环蛋白2，siderocalin和中性粒细胞脂环蛋白）是一种25 kDa糖基化的单链单体，是脂环蛋白家族中结合和运输亲脂性小分子的成员。 NGAL由活化的中性粒细胞释放，可形成二聚体和少量高级寡聚体，并与基质金属蛋白酶9（MMP-9，明胶酶B）形成复合物。在炎症或癌症中，多种上皮细胞中NGAL的低水平表达可能增加。缺血或肾毒性肾脏损伤可显着增加NGAL在肾脏中的表达。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

NGAL Human ELISA KitRepresentative standard curve for Human NGAL ELISA

技术参数

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NGAL Human ELISA Kit ELISA试剂盒,NGAL Human ELISA Kit/人NGAL ELISA试剂盒

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