TNF beta Human ELISA Kit ELISA试剂盒,TNF beta Human ELISA Kit/人TNF β ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

TNF beta Human ELISA Kit ELISA试剂盒,TNF beta Human ELISA Kit/人TNF β ELISA试剂盒

2024-10-23

TNF beta Human ELISA Kit/人TNF β ELISA试剂盒

货号：BMS202,BMS202TEN

规格：96 Tests,10X96 Tests

价格：6120,37859

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	4.6 pg/mL
检测范围	15.6-1,000 pg/mL
样本类型/体积	血清 100 μL 血浆 100 μL 上清液 100 μL
操作时间	1 hr 20 min
测试时间	4 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	10.2%
批内变异系数（CV）	8%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 对照 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	TNF beta
Protein aliases	LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, Lymphotoxin-alpha, TNF superfamily, member 1, tumor necrosis factor beta, tumor necrosis factor ligand 1E
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	LT, LTA, TNFB, TNFSF1, TNLG1E
Gene ID	(Human) 4049
Gene symbol	LTA
UniProt ID	(Human) P01374

The Human Tumor Necrosis Factor beta (Hu TNFβ) ELISA quantitates Hu TNFβ in human serum, plasma, urine, synovial fluid, amniotic fluid, cell culture supernatants, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu TNFβ.人肿瘤坏死因子β（人TNFβ）ELISA定量检测人血清，血浆，尿液，滑液，羊水，细胞培养上清液或其他体液中的人TNFβ。该检测试剂盒将只识别天然和重组的人TNFβ。Principle of the method 方法原理The Human TNFβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人TNFβ固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品或对照添加到这些孔中，并与固定的（捕获）抗体结合。通过将第二（检测）抗体与与捕获抗体不同的表位上的靶标结合形成三明治。与酶缀合的抗体结合形成的三明治。经过孵育和洗涤步骤以去除未结合物质的微孔板后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.TNFβ是肿瘤坏死因子家族的成员，是淋巴细胞产生的细胞因子。该蛋白高度可诱导，分泌并与淋巴毒素-β形成异源三聚体，从而将淋巴毒素-α锚定在细胞表面。该蛋白还介导多种炎症，免疫刺激和抗病毒反应，在发育过程中参与次级淋巴器官的形成，并在细胞凋亡中起作用。该基因的遗传变异与对4型麻风病和牛皮癣关节炎的易感性有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

TNF beta Human ELISA KitRepresentative standard curve for Human TNF beta ELISA

技术参数

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TNF beta Human ELISA Kit ELISA试剂盒,TNF beta Human ELISA Kit/人TNF β ELISA试剂盒

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