CD40L (Soluble) Mouse ELISA Kit ELISA试剂盒,CD40L (Soluble) Mouse ELISA Kit/小鼠CD40L（可溶）ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

CD40L (Soluble) Mouse ELISA Kit ELISA试剂盒,CD40L (Soluble) Mouse ELISA Kit/小鼠CD40L（可溶）ELISA试剂盒

2024-10-23

CD40L (Soluble) Mouse ELISA Kit/小鼠CD40L（可溶）ELISA试剂盒

货号：BMS6010,BMS6010TEN

规格：96 Tests,10X96 Tests

价格：6120,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：小鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.14 ng/mL
检测范围	0.31-20.0 ng/mL
样本类型/体积	血清 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	11.1%
批内变异系数（CV）	6.5%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	CD154 (CD40 Ligand)
Protein aliases	CD154, CD40 ligand, membrane form, soluble form, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5
物种（Tested）	小鼠
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	CD154, CD40-L, Cd40l, Cd40lg, gp39, HIGM1, IGM, IMD3, Ly-62, Ly62, T-BAM, Tnfsf5, TRAP
Gene ID	(Mouse) 21947
Gene symbol	Cd40lg
UniProt ID	(Mouse) P27548

The Mouse CD40 Ligand (Ms CD40L) ELISA quantitates Ms CD40L in mouse serum, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms CD40L.小鼠CD40配体（小鼠CD40L）ELISA定量检测小鼠血清或细胞培养基中的小鼠CD40L。该检测试剂盒将专门识别天然和重组小鼠CD40L。Principle of the method 方法原理The Mouse CD40L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠CD40L固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.CD40配体（CD40-L）或CD154是在T细胞表面表达的膜糖蛋白和分化抗原。在存在细胞因子的情况下，CD40配体刺激B细胞增殖和所有免疫球蛋白同种型的分泌。已经证明CD40配体在外周血单核细胞中诱导细胞因子的产生和杀肿瘤活性。它还共同刺激活化的T细胞增殖，并伴随着IFN-γ，TNF-α和IL-2的产生。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

CD40L (Soluble) Mouse ELISA KitRepresentative standard curve for Mouse soluble CD40 Ligand ELISA

技术参数

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CD40L (Soluble) Mouse ELISA Kit ELISA试剂盒,CD40L (Soluble) Mouse ELISA Kit/小鼠CD40L（可溶）ELISA试剂盒

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