VEGF Receptor 3/FLT4 Human ELISA Kit ELISA试剂盒,VEGF Receptor 3/FLT4 Human ELISA Kit/人VEGF 受体3/FLT4 ELISA试剂盒

2024-10-23

VEGF Receptor 3/FLT4 Human ELISA Kit/人VEGF 受体3/FLT4 ELISA试剂盒

货号:BMS2064,BMS2064TEN

规格:96 Tests,10X96 Tests

价格:6139,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.03 ng/mL

检测范围0.6-40.0 ng/mL

样本类型/体积

血清 20 μL

血浆 20 μL

上清液 20 μL

操作时间

1 hr 20 min

测试时间

3 hr 30 min

Homogenous (no wash)

No

批间变异系数(CV)5%
批内变异系数(CV)5.7%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameVEGF Receptor 3
Protein aliasesFLT-4, Fms-like tyrosine kinase 4, fms-related tyrosine kinase 4, Tyrosine-protein kinase receptor FLT4, Vascular endothelial growth factor receptor 3, VEGFR-3

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesFLT-4, FLT4, FLT41, LMPH1A, PCL, VEGFR-3, VEGFR3
Gene ID(Human) 2324
Gene symbolFLT4
UniProt ID(Human) P35916
The Human Vascular Endothelial Growth Factor Receptor 3/FLT4 (Hu VEGFR3) ELISA quantitates Hu VEGFR3 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VEGFR3.人血管内皮生长因子受体3 / FLT4(人VEGFR3)ELISA定量了人血清,血浆,缓冲液或细胞培养基中的人VEGFR3。 该检测试剂盒将专门识别天然和重组人VEGFR3。Principle of the method 方法原理The Human VEGFR3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人VEGFR3固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues. Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3. In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos. Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5. Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.血管内皮生长因子是胚胎中血管发育和成人组织中血管生成的关键调节剂。 与VEGF不同,相关的VEGFC通过其特异性的淋巴管内皮受体VEGFR-3刺激淋巴管的生长。 1998年,杜蒙(Dumont)表明,小鼠中VEGFR-3基因的靶向失活导致早期胚胎中血管发育的缺陷。 发生血管生成和血管生成,但是大血管变得异常组织,内腔缺损,导致在胚胎第9.5天液体积聚在心包腔中并导致心血管衰竭。 因此,VEGFR3在淋巴管出现之前在胚性心血管系统的发育中具有重要作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

VEGF Receptor 3/FLT4 Human ELISA KitRepresentative standard curve for Human VEGF Receptor 3/Flt 4 ELISA

技术参数
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