MICA Human ELISA Kit ELISA试剂盒,MICA Human ELISA Kit/人MICA ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

MICA Human ELISA Kit ELISA试剂盒,MICA Human ELISA Kit/人MICA ELISA试剂盒

2024-10-23

MICA Human ELISA Kit/人MICA ELISA试剂盒

货号：BMS2302,BMS2302TEN

规格：96 Tests,10X96 Tests

价格：6120,34832

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	1.09 pg/mL
检测范围	1.6-100 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	4 hr
Homogenous (no wash)	No
批间变异系数（CV）	6.6%
批内变异系数（CV）	5.1%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品（2瓶） - 标准稀释液 - 生物素化检测抗体 -Streptavidin-HRP - HRP稀释液 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	MICA
Protein aliases	HLA class I antigen, MHC class I chain-related protein A, MHC class I polypeptide-related sequence A, MIC-A, MHC class I related sequence A, MICA, stress inducible class I homolog, truncated MHC class I polypeptide-related sequence A
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	MIC-A, MICA, PERB11.1
Gene ID	(Human) 100507436
Gene symbol	MICA
UniProt ID	(Human) Q29983

The Human MICA ELISA quantitates Hu MICA in human serum, plasma, cell lysates, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MICA.人MICA ELISA可以定量人血清，血浆，细胞裂解液或细胞培养基中的人MICA。该检测试剂盒将专门识别天然和重组人MICA。Principle of the method 方法原理The Human MICA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人MICA固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，该第二抗体与与捕获抗体不同的表位上的靶标结合。已将结合酶掺入测定中。经过孵育和洗涤步骤以去除未结合物质的微孔板后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Major histocompatibility complex (MHC) class I proteins are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. A related family, termed the MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. Both MICA and MICB mRNA are widely expressed in normal tissues, with MICA being present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.主要的组织相容性复合体（MHC）I类蛋白无处不在，并通过细胞毒性T细胞介导对细胞内抗原的识别。一个相关家族，称为MHC I类链相关（MIC）蛋白，被NK和T细胞上的一种受体NKG2D识别，并促进抗肿瘤活性。 MICA是MIC家族的一员，在许多肿瘤中广泛表达，而MICA / NKG2D相互作用被认为可刺激T淋巴细胞的抗肿瘤反应性。 MICA和MICB mRNA均在正常组织中广泛表达，而除神经系统外，几乎所有组织中都存在MICA，这表明MIC蛋白表达可能只是免疫系统抗肿瘤活性的一个组成部分。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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MICA Human ELISA Kit ELISA试剂盒,MICA Human ELISA Kit/人MICA ELISA试剂盒

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