AXL Human ELISA Kit ELISA试剂盒,AXL Human ELISA Kit/人AXL ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

AXL Human ELISA Kit ELISA试剂盒,AXL Human ELISA Kit/人AXL ELISA试剂盒

2024-10-23

AXL Human ELISA Kit/人AXL ELISA试剂盒

货号：BMS2286

规格：96 Tests

价格：6120

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	7.457 pg/mL
检测范围	31.25-2000 pg/mL
样本类型/体积	血清 10 μL 血浆 10 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	10%
批内变异系数（CV）	8.6%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品（2瓶） - 标准稀释液 - 生物素化检测抗体 -Streptavidin-HRP - HRP稀释液 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	AXL
Protein aliases	AXL oncogene, AXL transforming sequence/gene, Tyrosine-protein kinase receptor UFO
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	ARK, AXL, JTK11, Tyro7, UFO
Gene ID	(Human) 558
Gene symbol	AXL
UniProt ID	(Human) P30530

The Human AXL ELISA quantitates Hu AXL in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu AXL.人AXL ELISA可以定量人血清，血浆或细胞培养基中的人AXL。该检测试剂盒将专门识别天然和重组人AXL。Principle of the method 方法原理The Human AXL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人AXL固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，该第二抗体与与捕获抗体不同的表位上的靶标结合。已将结合酶掺入测定中。经过孵育和洗涤步骤以去除未结合物质的微孔板后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Axl is a member of the TAM receptor family, which also includes also MerTK and TYRO3. Axl contains protein kinase activity and is activated by dimerization and auto-phosphorylation upon ligand binding. In response to its ligands, Protein S and GAS6, Axl activates anti-inflammatory pathways (through SOCS-1 and SOCS-3), which limits TLR and cytokine signaling. This results in dampened inflammatory responses in macrophages and DCs. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.Axl是TAM受体家族的成员，该家族还包括MerTK和TYRO3。 Axl含有蛋白激酶活性，并在配体结合后被二聚化和自身磷酸化激活。响应其配体蛋白S和GAS6，Axl激活了抗炎途径（通过SOCS-1和SOCS-3），从而限制了TLR和细胞因子的信号传导。这导致巨噬细胞和DC的炎症反应减弱。 TAM受体也参与凋亡细胞的吞噬作用。缺乏所有三种TAM受体的小鼠具有几种退化性表型，与凋亡细胞和膜的去除效率低下有关（例如，在视网膜和雄性生殖道中），并发展出类似于系统性红斑狼疮的严重自身免疫表型，包括自动产生广谱-抗体。另外，Axl可能充当丝状病毒的推定进入受体。 Axl也用于鉴定人血液树突细胞的特定亚群，也称为Siglec 6 + / Axl +树突细胞。 Axl的细胞表达可以通过TLR配体（例如LPS或poly I：C）上调。通过膜形式的蛋白水解切割产生可溶性Axl。血浆水平升高可能表明炎症和癌症。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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AXL Human ELISA Kit ELISA试剂盒,AXL Human ELISA Kit/人AXL ELISA试剂盒

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