Cyclic AMP Competitive ELISA Kit/循环AMP竞争性ELISA试剂盒

货号：EMSCAMPL

规格：96 tests

价格：0

产品类型：ELISA检测

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.037 pmol/mL
检测范围	0.078-20 pmol/mL
样本类型/体积	细胞裂解液 100 μL 组织匀浆 100 μL
操作时间	1 hr 20 min
测试时间	3 hr
Homogenous (no wash)	No
批间变异系数（CV）	7.1%
批内变异系数（CV）	6.5%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 检测抗体 - AP共轭物 - 中和试剂 -三乙胺 -醋酸酐 - 标准品 - 20X洗涤液 - pNpp底物溶液 - 终止液 - 封板膜
储存温度	-20°C
Protein name	cAMP
Protein aliases	Cyclic AMP
物种（Tested）	人
试剂盒类型	Competitive ELISA
标记（染料）	AP

The cyclic AMP Competitive ELISA quantitates cAMP in cell or tissue lysates. The assay will exclusively recognize both natural and recombinant cyclic AMP.环状AMP竞争性ELISA定量分析细胞或组织裂解物中的cAMP。 该检测试剂盒将专门识别天然和重组环状AMP。Principle of the method 方法原理The cyclic AMP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen.环状AMP固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，该第二抗体与与捕获抗体不同的表位上的靶标结合。 已将结合酶掺入测定中。 经过孵育和洗涤步骤以去除未结合物质的微孔板后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样本中存在的目标浓度成反比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Cyclic adenosine monophosphate (cAMP) plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals. The G-Protein Coupled Receptors (GPCR) is the largest family of cell surface receptors. They can be activated by different ligands, such as neurotransmitters, hormones, ions, small molecules, peptides, and other physiological signaling molecules. Typically, the binding of the ligands to its receptor resulting in the activation of G-proteins, in return, activates the effector adenylyl cyclase evoking the production of cAMP. The activation of a protein kinase by cAMP results in the phosphorylation of substrate proteins. Currently successful drugs in marketing have been developed to target these receptors. Among the GPCRs, ~367 receptors are potential drug development targets, but only about 20 have been used to generate therapeutically and commercially successful drugs so far. Because the involvement of cAMP can amplify the response of the ligand binding, the second messenger cAMP has been largely employed to monitor the activation of the GPCR to facilitate the therapeutic drug discovery.环磷酸一腺苷（cAMP）作为细胞内第二信使，在跟随许多细胞外信号的转导事件中起着关键作用。 G蛋白偶联受体（GPCR）是细胞表面受体的最大家族。它们可以被不同的配体激活，例如神经递质，激素，离子，小分子，肽和其他生理信号分子。通常，配体与其受体的结合导致G蛋白的激活，进而激活效应腺苷酸环化酶，从而引起cAMP的产生。 cAMP激活蛋白激酶会导致底物蛋白磷酸化。目前已经开发出了成功的靶向这些受体的药物。在GPCR中，约367种受体是潜在的药物开发目标，但到目前为止，仅约20种受体已用于产生治疗上和商业上成功的药物。由于cAMP的参与可以放大配体结合的反应，因此第二信使cAMP已被广泛用于监测GPCR的激活，以促进治疗药物的发现。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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