Prostaglandin E2 Human Competitive ELISA Kit/人前列腺素E2竞争性ELISA试剂盒

货号：EHPGE2

规格：96 tests

价格：7304

产品类型：ELISA检测

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	13.4 pg/mL
检测范围	39.1-2500 pg/mL
样本类型/体积	唾液 10 µL 血清 10 µL 上清液 100 µL 尿液 10 µL 全血 10 µL
操作时间	1 hr 20 min
测试时间	2 hr 45 min
Homogenous (no wash)	No
批间变异系数（CV）	4%
批内变异系数（CV）	10.7%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 测定稀释液 - 检测抗体 -AP共轭物 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	Prostaglandin E2
Protein aliases	C20H32O5, PGE2
物种（Tested）	人
试剂盒类型	Competitive ELISA
标记（染料）	AP
检测抗体偶联物	AP

The Human Prostaglandin E2 (Hu PGE2) ELISA quantitates Hu PGE2 in human serum, whole blood, saliva, urine or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PGE2.人前列腺素E2（人PGE2）ELISA定量了人血清，全血，唾液，尿液或细胞培养基中的人PGE2。 该检测试剂盒将专门识别天然和重组人PGE2。Principle of the method 方法原理The Human PGE2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen.人PGE2固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样本中存在的目标浓度成反比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Prostoglandin E2 (PGE2) is a potent lipid mediator produced by metabolism of arachidonic acid via the cyclooxygenase (COX) pathway. Following cellular stimulation, arachidonic acid is hydrolyzed from phospholipids stores by one of a family of phospholipase A2 enzymes and converted to the unstable endoperoxide PGH2 by either COX 1 or COX 2. PGH2 is isomerized to PGE2 by one of 3 distinct enzymes microsomal PGES 1 (mPGES 1), mPGES 2, or a cytosolic enzyme, cPGES. PGE2 is active in a number of physiological systems and settings including inflammation, immune regulation, generation of fever, pain perception, protection of the gastric muscosa, fertility and parturition, as well as sodium and water retention. The effects of PGE2 are transduced by 4 subtypes of G protein coupled receptors designated EP1, EP2, EP3, EP4, with Kd values for PGE2 ranging from 1 10 nM. PGE2 is rapidly metabolized in vivo by the prostaglandin 15 dehydrogenase pathway (15 hydroxy PGDH) to the inactive metabolite 13,14 dihydro 15 keto PGE2. The half life of PGE2 in the circulatory system is ~30 seconds and normal plasma levels are 3 12 pg/mL.前列腺素E2（PGE2）是一种有效的脂质介体，由花生四烯酸经由环氧合酶（COX）途径代谢而产生。在细胞刺激后，花生四烯酸被磷脂酶A2酶家族中的一种从磷脂库中水解出来，并被COX 1或COX 2转化为不稳定的过氧化物PGH2。PGH2被微粒体PGES 1的3种不同酶之一异构化为PGE2（ mPGES 1），mPGES 2或胞质酶cPGES。 PGE2在许多生理系统和环境中均具有活性，包括炎症，免疫调节，发烧，疼痛感，保护胃粘膜，生育力和分娩以及钠和水water留。 PGE2的作用可通过G蛋白偶联受体的4个亚型（分别称为EP1，EP2，EP3，EP4）进行转导，PGE2的Kd值范围为1 10 nM。 PGE2在体内通过前列腺素15脱氢酶途径（15羟基PGDH）迅速代谢为非活性代谢物13,14二氢15酮PGE2。 PGE2在循环系统中的半衰期约为30秒，正常血浆水平为3 12 pg / mL。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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