Progesterone Competitive ELISA Kit/孕酮竞争性ELISA试剂盒

货号：EIAP4C21,EIAP4C21X10

规格：96 tests,10X96 tests

价格：7311,52892

产品类型：ELISA检测

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	47.9 pg/mL
检测范围	50-3200 pg/mL
样本类型/体积	粪便提取物 50 µL 上清液 50 µL 尿液 12.5 µL
操作时间	1 hr 20 min
测试时间	2 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	5.7%
批内变异系数（CV）	3.9%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 测定稀释液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	Progesterone
Protein aliases	OHP, 11-alpha-hydroxyprogesterone, Hydroxyprogesterone alpha, hydroxyprogesterone 11, Progesterone-11a-hemisuccinate, Agolutin, Crinone, Luteohormone, Pregn-4-ene-3, 20-dione, C21H30O2
物种（Tested）	Chemical
试剂盒类型	Competitive ELISA
标记（染料）	HRP
检测抗体偶联物	HRP

The Progesterone ELISA quantitates progesterone in urine, dried fecal extracts or cell culture medium.孕酮ELISA定量尿液，干燥的粪便提取物或细胞培养基中的孕酮。Principle of the method 方法原理The Progesterone Competitive ELISA research-use-only kit is designed to quantitatively measure Progesterone present in extracted dried fecal samples, urine, and tissue culture media samples. A progesterone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A progesterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to progesterone to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound progesterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.孕酮竞争性ELISA试剂盒用于定量测量提取的干燥粪便样品，尿液和组织培养基样品中存在的孕酮。 提供孕酮标准品以生成测定的标准曲线，所有样品应从标准曲线中读出。 将标准品或稀释的样品吸移到涂有抗体的透明微量滴定板中，以捕获小鼠抗体。 将黄体酮-过氧化物酶结合物添加到孔中的标准品和样品中。 通过向每个孔中添加孕激素单克隆抗体来引发结合反应。 温育2小时后，洗涤板并加入底物。 底物与结合的孕酮-过氧化物酶缀合物反应。 短暂孵育后，终止反应，并在能够在450 nm处测量的微量滴定板读数器中检测所产生颜色的强度。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Progesterone is the major progestational steroid, secreted primarily by the corpus luteum and the placenta. Progesterone binds to the progesterone receptor, resulting in dissociation of heat shock proteins, receptor phosphorylation, and transcription activation through direct or indirect interaction with transcription factors. Progesterone acts on the uterus, the mammary glands and the brain. It is required in embryo implantation, pregnancy maintenance, and the development of mammary tissue for milk production. Progesterone, converted from pregnenolone, also serves as an intermediate in the biosynthesis of gonadal steroid hormones and adrenal corticosteroids. Therapeutic progesterone is a synthetic form of the endogenous hormone progesterone. This agent exerts inhibitory effects on estrogens by decreasing the number of estrogen receptors and increasing its metabolism to inactive metabolites. [National Center for Biotechnology Information. PubChem Compound Database; CID=5994]孕酮是主要的孕激素，主要由黄体和胎盘分泌。 孕酮与孕激素受体结合，导致热休克蛋白解离，受体磷酸化以及通过与转录因子直接或间接相互作用而引起的转录激活。 黄体酮作用于子宫，乳腺和大脑。 在胚胎植入，怀孕维持和乳汁发育乳汁生产中需要它。 从孕烯醇酮转化的孕酮，也可以作为性腺类固醇激素和肾上腺皮质类固醇生物合成的中间体。 孕激素是内源性孕激素的合成形式。 该药物通过减少雌激素受体的数量并增加其代谢成无活性代谢物的作用，对雌激素产生抑制作用。 [国家生物技术信息中心。 PubChem化合物数据库； CID = 5994]For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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